Contractile imbalance of human cardiac microtissues on the mechanical hybrid filamentous matrices

Chenyan Wang, Sangmo Koo, Plansky Hoang, Costas Grigoropoulos, Kevin E. Healy, Zhen Ma

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: 3D cardiac microtissues developed from human induced pluripotent stem cells (hiPSC-µCTs) have offered a unique opportunity to study mechanical-induced cardiac adaptation and malfunctions. Optimal mechanical load was critical for the maintenance and maturation of hiPSC-µCTs with highly organized sarcomeres. In contrast, combination of mechanical overload and genetic deficiency has been shown to induce contractile deficits of the hiPSC-µCTs 1 . These studies indicated that the mechanical stress incorporates key niche elements that regulates of physiological functions and pathological phenotypes of hiPSC-µCTs. The majority of current engineered hiPSC-µCTs still heavily focus on biomimetic designs to create physiological relevant tissue models. In this study, we established a 3D cardiac tissue model based on synthetic filamentous matrices with fully artificial designs that present complex mechanical environment to the hiPSC-µCTs. In the filamentous matrices, synthetic fibers served as not only the backbone to organize a 3D cardiac microtissue, but also the physical cues to modulate the tissue mechanics. By positioning fibers with different diameters within one matrix, we created different designs of mechanical hybrid matrices, thus we can investigate the contractile behaviors and adaptive ability of hiPSC-µCTs to the complex mechanical environments.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

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  • Cite this

    Wang, C., Koo, S., Hoang, P., Grigoropoulos, C., Healy, K. E., & Ma, Z. (2019). Contractile imbalance of human cardiac microtissues on the mechanical hybrid filamentous matrices. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.