Continuous noninvasive in vivo monitoring of intravascular plasma volume and hematocrit changes in response to blood removal and fluid replacement in a rat model

Bin Deng, Evan Kastner, P. Dent, J. Goodisman, J. Chaiken

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Scopus citations

Abstract

We report a new algorithm and measurement system that permits simultaneous monitoring of the hematocrit and plasma volume fraction of blood within the intravascular space of an optically probed volume of skin. The system involves probing with a near infrared laser and simultaneously collecting the Rayleigh and Mie scattered light as one raw signal and the undifferentiated Raman and fluorescence emission as the second raw signal. Those two physically independent raw signals and six parameters that can be obtained by either direct calculation or empirical calibration permit monitoring of the blood in rat paws. We tested a device based on the algorithm in the context of improving detection of blood loss for people with an early undiagnosed internal hemorrhage via real-time monitoring of signal changes with direct correlation to hematocrit. We performed experiments monitoring rat paw skin in vivo while removing blood, centrally or peripherally, and then adding replacement fluids such as Normocarb and blood. Blood removal itself elicits a predictable and consistent response, decreasing hematocrit and increasing relative plasma volume, that depends on the rate and location of removal, the total amount of blood removed, the location of monitoring, and possibly other factors as yet unknown. Similarly, replacing the blood with whole blood vs. saline consistently produces a rational range of responses. Calibration across subjects and the measurement of absolute hematocrit will also be discussed.

Original languageEnglish (US)
Title of host publicationAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XII
PublisherSPIE
ISBN (Print)9780819498489
DOIs
StatePublished - Jan 1 2014
EventAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XII - San Francisco, CA, United States
Duration: Feb 2 2014Feb 4 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8935
ISSN (Print)1605-7422

Other

OtherAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XII
CountryUnited States
CitySan Francisco, CA
Period2/2/142/4/14

    Fingerprint

Keywords

  • Continuous
  • Hematocrit
  • Hemorrhage
  • In vivo
  • Noninvasive
  • Plasma volume
  • Rat model

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Deng, B., Kastner, E., Dent, P., Goodisman, J., & Chaiken, J. (2014). Continuous noninvasive in vivo monitoring of intravascular plasma volume and hematocrit changes in response to blood removal and fluid replacement in a rat model. In Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XII [893526] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8935). SPIE. https://doi.org/10.1117/12.2043976