Consumption of florfenicol-medicated feed alters the composition of the channel catfish intestinal microbiota including enriching the relative abundance of opportunistic pathogens

Erlong Wang, Zihao Yuan, Kaiyu Wang, Dongya Gao, Zhanjian "John" Liu, Mark R. Liles

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Antibiotics are frequently used in feed to control bacterial diseases in aquaculture settings. In addition to the intended purpose of controlling diseases, applications of medicated feed may significantly change the composition of the gut microbiota, which in turn may have impact on host-pathogen interactions. However, nothing is known concerning the effects of antibiotics on microbiota in the gut channel catfish (Ictalurus punctatus), the most important aquaculture species in the United States. In this study, we determined the impact of medicated feed containing florfenicol on microbiota in the gut of catfish at various times post-feeding. Through analysis of bacterial 16S rRNA gene amplicons, we found that the medicated feed had a dramatic impact on the composition of the gut microbiota, with significant enrichment of Plesiomonas spp., accounting for 66% of all gut bacteria. Along with other related species, Proteobacteria taxa accounted for 93% of all microbiota by day 10; in contrast, the gut microbiota of fish receiving non-medicated feed harbored a bacterial assemblage that had greater ribotype richness and a more even distribution (P <.05), with <10% Proteobacteria relative abundance. Moreover, florfenicol-medicated feed resulted in an increased relative abundance of potential opportunistic pathogens including Plesiomonas and Aeromonas species, which may have detrimental impacts on fish health. This study provided insights into the specific bacterial taxa within the channel catfish intestinal microbiome that were impacted by florfenicol-medicated feed and suggests that the abuse of this and other antibiotics by the aquaculture industry may induce the relative abundance of potentially pathogenic gut microorganisms.

Original languageEnglish (US)
Pages (from-to)111-118
Number of pages8
JournalAquaculture
Volume501
DOIs
StatePublished - Feb 25 2019

Fingerprint

feed composition
florfenicol
medicated feeds
Ictalurus punctatus
intestinal microorganisms
antibiotics
relative abundance
pathogen
Plesiomonas
aquaculture
pathogens
host-pathogen interaction
Proteobacteria
bacterial disease
aquaculture industry
fish
digestive system
ribotypes
microorganism
fish health

Keywords

  • Antibiotics
  • Bacterial diversity
  • Fish
  • Florfenicol
  • Gut microbiota
  • Ribotype

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Consumption of florfenicol-medicated feed alters the composition of the channel catfish intestinal microbiota including enriching the relative abundance of opportunistic pathogens. / Wang, Erlong; Yuan, Zihao; Wang, Kaiyu; Gao, Dongya; Liu, Zhanjian "John"; Liles, Mark R.

In: Aquaculture, Vol. 501, 25.02.2019, p. 111-118.

Research output: Contribution to journalArticle

@article{e58d6f6d92e44716b19feda89b10c035,
title = "Consumption of florfenicol-medicated feed alters the composition of the channel catfish intestinal microbiota including enriching the relative abundance of opportunistic pathogens",
abstract = "Antibiotics are frequently used in feed to control bacterial diseases in aquaculture settings. In addition to the intended purpose of controlling diseases, applications of medicated feed may significantly change the composition of the gut microbiota, which in turn may have impact on host-pathogen interactions. However, nothing is known concerning the effects of antibiotics on microbiota in the gut channel catfish (Ictalurus punctatus), the most important aquaculture species in the United States. In this study, we determined the impact of medicated feed containing florfenicol on microbiota in the gut of catfish at various times post-feeding. Through analysis of bacterial 16S rRNA gene amplicons, we found that the medicated feed had a dramatic impact on the composition of the gut microbiota, with significant enrichment of Plesiomonas spp., accounting for 66{\%} of all gut bacteria. Along with other related species, Proteobacteria taxa accounted for 93{\%} of all microbiota by day 10; in contrast, the gut microbiota of fish receiving non-medicated feed harbored a bacterial assemblage that had greater ribotype richness and a more even distribution (P <.05), with <10{\%} Proteobacteria relative abundance. Moreover, florfenicol-medicated feed resulted in an increased relative abundance of potential opportunistic pathogens including Plesiomonas and Aeromonas species, which may have detrimental impacts on fish health. This study provided insights into the specific bacterial taxa within the channel catfish intestinal microbiome that were impacted by florfenicol-medicated feed and suggests that the abuse of this and other antibiotics by the aquaculture industry may induce the relative abundance of potentially pathogenic gut microorganisms.",
keywords = "Antibiotics, Bacterial diversity, Fish, Florfenicol, Gut microbiota, Ribotype",
author = "Erlong Wang and Zihao Yuan and Kaiyu Wang and Dongya Gao and Liu, {Zhanjian {"}John{"}} and Liles, {Mark R.}",
year = "2019",
month = "2",
day = "25",
doi = "10.1016/j.aquaculture.2018.11.019",
language = "English (US)",
volume = "501",
pages = "111--118",
journal = "Aquaculture",
issn = "0044-8486",
publisher = "Elsevier",

}

TY - JOUR

T1 - Consumption of florfenicol-medicated feed alters the composition of the channel catfish intestinal microbiota including enriching the relative abundance of opportunistic pathogens

AU - Wang, Erlong

AU - Yuan, Zihao

AU - Wang, Kaiyu

AU - Gao, Dongya

AU - Liu, Zhanjian "John"

AU - Liles, Mark R.

PY - 2019/2/25

Y1 - 2019/2/25

N2 - Antibiotics are frequently used in feed to control bacterial diseases in aquaculture settings. In addition to the intended purpose of controlling diseases, applications of medicated feed may significantly change the composition of the gut microbiota, which in turn may have impact on host-pathogen interactions. However, nothing is known concerning the effects of antibiotics on microbiota in the gut channel catfish (Ictalurus punctatus), the most important aquaculture species in the United States. In this study, we determined the impact of medicated feed containing florfenicol on microbiota in the gut of catfish at various times post-feeding. Through analysis of bacterial 16S rRNA gene amplicons, we found that the medicated feed had a dramatic impact on the composition of the gut microbiota, with significant enrichment of Plesiomonas spp., accounting for 66% of all gut bacteria. Along with other related species, Proteobacteria taxa accounted for 93% of all microbiota by day 10; in contrast, the gut microbiota of fish receiving non-medicated feed harbored a bacterial assemblage that had greater ribotype richness and a more even distribution (P <.05), with <10% Proteobacteria relative abundance. Moreover, florfenicol-medicated feed resulted in an increased relative abundance of potential opportunistic pathogens including Plesiomonas and Aeromonas species, which may have detrimental impacts on fish health. This study provided insights into the specific bacterial taxa within the channel catfish intestinal microbiome that were impacted by florfenicol-medicated feed and suggests that the abuse of this and other antibiotics by the aquaculture industry may induce the relative abundance of potentially pathogenic gut microorganisms.

AB - Antibiotics are frequently used in feed to control bacterial diseases in aquaculture settings. In addition to the intended purpose of controlling diseases, applications of medicated feed may significantly change the composition of the gut microbiota, which in turn may have impact on host-pathogen interactions. However, nothing is known concerning the effects of antibiotics on microbiota in the gut channel catfish (Ictalurus punctatus), the most important aquaculture species in the United States. In this study, we determined the impact of medicated feed containing florfenicol on microbiota in the gut of catfish at various times post-feeding. Through analysis of bacterial 16S rRNA gene amplicons, we found that the medicated feed had a dramatic impact on the composition of the gut microbiota, with significant enrichment of Plesiomonas spp., accounting for 66% of all gut bacteria. Along with other related species, Proteobacteria taxa accounted for 93% of all microbiota by day 10; in contrast, the gut microbiota of fish receiving non-medicated feed harbored a bacterial assemblage that had greater ribotype richness and a more even distribution (P <.05), with <10% Proteobacteria relative abundance. Moreover, florfenicol-medicated feed resulted in an increased relative abundance of potential opportunistic pathogens including Plesiomonas and Aeromonas species, which may have detrimental impacts on fish health. This study provided insights into the specific bacterial taxa within the channel catfish intestinal microbiome that were impacted by florfenicol-medicated feed and suggests that the abuse of this and other antibiotics by the aquaculture industry may induce the relative abundance of potentially pathogenic gut microorganisms.

KW - Antibiotics

KW - Bacterial diversity

KW - Fish

KW - Florfenicol

KW - Gut microbiota

KW - Ribotype

UR - http://www.scopus.com/inward/record.url?scp=85056453922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056453922&partnerID=8YFLogxK

U2 - 10.1016/j.aquaculture.2018.11.019

DO - 10.1016/j.aquaculture.2018.11.019

M3 - Article

VL - 501

SP - 111

EP - 118

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

ER -