Construction of a vnfH::lacZ fusion and study of expression from the vnfH promoter of the vanadium-dependent nitrogen fixation pathway in Azotobacter vinelandii

Ramesh Raina; Umesh K. Bageshwar; H. K. Das

doi:10.1111/j.1574-6968.1992.tb05508.x

Construction of a vnfH::lacZ fusion and study of expression from the vnfH promoter of the vanadium-dependent nitrogen fixation pathway in Azotobacter vinelandii

Ramesh Raina, Umesh K. Bageshwar, H. K. Das

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A lac fusion of the vnfH promoter of Azotobacter vinelandii has been constructed. An upstream sequence seems to be necessary for the activity of the promoter. The repression of expression of this promoter by fixed nitrogen is several-fold lower compared to that of the nifH promoter. Vanadium has no role in the expression of the nifH or the vnfH promoter. Molybdenum is essential for the nifH promoter and represses the vnfH promoter. Tungsten can substitute molybdenum in its regulatory role. ntrA and vnfA are essential for the expression of vnfH, but ntrC has no role.

Original language	English (US)
Pages (from-to)	169-173
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	98
Issue number	1-3
DOIs	https://doi.org/10.1111/j.1574-6968.1992.tb05508.x
State	Published - Nov 1 1992
Externally published	Yes

Keywords

Ammonia
Azotobacter vinelandii
Gene expression
Molybdenum
Vanadium
lac Fusion
vnfH Promoter

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1111/j.1574-6968.1992.tb05508.x

Cite this

@article{96955a61dde34ad58b400b393231d1a4,

title = "Construction of a vnfH::lacZ fusion and study of expression from the vnfH promoter of the vanadium-dependent nitrogen fixation pathway in Azotobacter vinelandii",

abstract = "A lac fusion of the vnfH promoter of Azotobacter vinelandii has been constructed. An upstream sequence seems to be necessary for the activity of the promoter. The repression of expression of this promoter by fixed nitrogen is several-fold lower compared to that of the nifH promoter. Vanadium has no role in the expression of the nifH or the vnfH promoter. Molybdenum is essential for the nifH promoter and represses the vnfH promoter. Tungsten can substitute molybdenum in its regulatory role. ntrA and vnfA are essential for the expression of vnfH, but ntrC has no role.",

keywords = "Ammonia, Azotobacter vinelandii, Gene expression, Molybdenum, Vanadium, lac Fusion, vnfH Promoter",

author = "Ramesh Raina and Bageshwar, {Umesh K.} and Das, {H. K.}",

note = "Funding Information: We are obliged to A. Puhler, D.R. Dean, P.E. Bishop and C. Kennedy for the strains. R.R. and U.K.B. are obliged to the University Grants Commission and the Council of Scientific and Industrial Research, India, for fellowships. This work has been supported by the Department of Science and Technology and the Department of Biotechnology, India.",

year = "1992",

month = nov,

day = "1",

doi = "10.1111/j.1574-6968.1992.tb05508.x",

language = "English (US)",

volume = "98",

pages = "169--173",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "1-3",

}

TY - JOUR

T1 - Construction of a vnfH::lacZ fusion and study of expression from the vnfH promoter of the vanadium-dependent nitrogen fixation pathway in Azotobacter vinelandii

AU - Raina, Ramesh

AU - Bageshwar, Umesh K.

AU - Das, H. K.

N1 - Funding Information: We are obliged to A. Puhler, D.R. Dean, P.E. Bishop and C. Kennedy for the strains. R.R. and U.K.B. are obliged to the University Grants Commission and the Council of Scientific and Industrial Research, India, for fellowships. This work has been supported by the Department of Science and Technology and the Department of Biotechnology, India.

PY - 1992/11/1

Y1 - 1992/11/1

N2 - A lac fusion of the vnfH promoter of Azotobacter vinelandii has been constructed. An upstream sequence seems to be necessary for the activity of the promoter. The repression of expression of this promoter by fixed nitrogen is several-fold lower compared to that of the nifH promoter. Vanadium has no role in the expression of the nifH or the vnfH promoter. Molybdenum is essential for the nifH promoter and represses the vnfH promoter. Tungsten can substitute molybdenum in its regulatory role. ntrA and vnfA are essential for the expression of vnfH, but ntrC has no role.

AB - A lac fusion of the vnfH promoter of Azotobacter vinelandii has been constructed. An upstream sequence seems to be necessary for the activity of the promoter. The repression of expression of this promoter by fixed nitrogen is several-fold lower compared to that of the nifH promoter. Vanadium has no role in the expression of the nifH or the vnfH promoter. Molybdenum is essential for the nifH promoter and represses the vnfH promoter. Tungsten can substitute molybdenum in its regulatory role. ntrA and vnfA are essential for the expression of vnfH, but ntrC has no role.

KW - Ammonia

KW - Azotobacter vinelandii

KW - Gene expression

KW - Molybdenum

KW - Vanadium

KW - lac Fusion

KW - vnfH Promoter

UR - http://www.scopus.com/inward/record.url?scp=0026440535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026440535&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.1992.tb05508.x

DO - 10.1111/j.1574-6968.1992.tb05508.x

M3 - Article

AN - SCOPUS:0026440535

SN - 0378-1097

VL - 98

SP - 169

EP - 173

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1-3

ER -

Construction of a vnfH::lacZ fusion and study of expression from the vnfH promoter of the vanadium-dependent nitrogen fixation pathway in Azotobacter vinelandii

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this