Construction of a vnfH::lacZ fusion and study of expression from the vnfH promoter of the vanadium-dependent nitrogen fixation pathway in Azotobacter vinelandii

Ramesh Raina, Umesh K. Bageshwar, H. K. Das

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A lac fusion of the vnfH promoter of Azotobacter vinelandii has been constructed. An upstream sequence seems to be necessary for the activity of the promoter. The repression of expression of this promoter by fixed nitrogen is several-fold lower compared to that of the nifH promoter. Vanadium has no role in the expression of the nifH or the vnfH promoter. Molybdenum is essential for the nifH promoter and represses the vnfH promoter. Tungsten can substitute molybdenum in its regulatory role. ntrA and vnfA are essential for the expression of vnfH, but ntrC has no role.

Original languageEnglish (US)
Pages (from-to)169-173
Number of pages5
JournalFEMS Microbiology Letters
Volume98
Issue number1-3
DOIs
StatePublished - Nov 1 1992
Externally publishedYes

Keywords

  • Ammonia
  • Azotobacter vinelandii
  • Gene expression
  • Molybdenum
  • Vanadium
  • lac Fusion
  • vnfH Promoter

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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