Conjugated polyene fatty acids as membrane probes: preliminary characterization

L. A. Sklar, Bruce S Hudson, R. D. Simoni

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The use of fluorescent conjugated polyenoic fatty acids as probes of membrane structure is introduced. α and β parinaric acid (cis, trans, trans, cis, and all trans 9,11,13,15 octadecatetraenoic acid) and synthetic lecithins containing an α parinaric acid chain in position 2 are prepared and their absorption and fluorescence properties are determined. Phase transitions are detected as fluorecence changes at characteristic temperatures when either the free fatty acid probes or the labeled phospholipid probe are included in sonicated aqueous dispersions of L α dimyristoyl lecithin, L α dipalmitoyl lecithin, or L α distearoyl lecithin. The phase transitions are detected at about 23°C (dimyristoyl), 44°C (dipalmitoyl), and 53°C (distearoyl lecithin). Binding of α parinaric acid to bovine serum albumin is measured by shifts in the absorption spectrum and enhanced quantum yield of the fatty acid upon binding and by energy transfer between 2 tryptophyl residues in bovine serum albumin and α parinaric acid. Approximately six binding sites are detected. Other applications of these probe molecules, including phase transitions of phospholipid/cholesterol dispersions, induced circular dichroism of parinaric acid bound to albumin, and biosynthetic incorporation of parinaric acid into biological membranes, are discussed.

Original languageEnglish (US)
Pages (from-to)1649-1653
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number5
StatePublished - 1975
Externally publishedYes

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Polyenes
Lecithins
Fatty Acids
Membranes
Phase Transition
Bovine Serum Albumin
Phospholipids
Energy Transfer
Circular Dichroism
parinaric acid
Nonesterified Fatty Acids
Albumins
Fluorescence
Binding Sites
Cholesterol
Temperature

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Conjugated polyene fatty acids as membrane probes : preliminary characterization. / Sklar, L. A.; Hudson, Bruce S; Simoni, R. D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, No. 5, 1975, p. 1649-1653.

Research output: Contribution to journalArticle

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