Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids

Jia Liu, Carlos A. Castañeda, Bryan J. Wilkins, David Fushman, T. Ashton Cropp

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures.

Original languageEnglish (US)
Pages (from-to)5613-5616
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume20
Issue number19
DOIs
StatePublished - Oct 1 2010

Keywords

  • Aminoacyl-tRNA synthetase
  • Ubiquitin
  • Unnatural amino acid
  • tRNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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