Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids

Jia Liu; Carlos A. Castañeda; Bryan J. Wilkins; David Fushman; T. Ashton Cropp

doi:10.1016/j.bmcl.2010.08.049

Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids

Jia Liu, Carlos A. Castañeda, Bryan J. Wilkins, David Fushman, T. Ashton Cropp

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures.

Original language	English (US)
Pages (from-to)	5613-5616
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	20
Issue number	19
DOIs	https://doi.org/10.1016/j.bmcl.2010.08.049
State	Published - Oct 1 2010
Externally published	Yes

Keywords

Aminoacyl-tRNA synthetase
Ubiquitin
Unnatural amino acid
tRNA

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2010.08.049

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title = "Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids",

abstract = "Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures.",

keywords = "Aminoacyl-tRNA synthetase, Ubiquitin, Unnatural amino acid, tRNA",

author = "Jia Liu and Casta{\~n}eda, {Carlos A.} and Wilkins, {Bryan J.} and David Fushman and Cropp, {T. Ashton}",

note = "Funding Information: The authors are grateful to Professor Peter Schultz (The Scripps Research Institute) for materials. Financial support from the National Institutes of Health ( GM084396 and GM065334 ) and the National Science Foundation ( CHE-0848398 ) is greatly acknowledged. Funding information: This work was supported by the National Institutes of Health (GM084396 to T.A.C. and GM065334 to D.F.) and the National Science Foundation (CHE-0848398 to T.A.C.). ",

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doi = "10.1016/j.bmcl.2010.08.049",

language = "English (US)",

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journal = "Bioorganic and Medicinal Chemistry Letters",

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T1 - Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids

AU - Liu, Jia

AU - Castañeda, Carlos A.

AU - Wilkins, Bryan J.

AU - Fushman, David

AU - Cropp, T. Ashton

N1 - Funding Information: The authors are grateful to Professor Peter Schultz (The Scripps Research Institute) for materials. Financial support from the National Institutes of Health ( GM084396 and GM065334 ) and the National Science Foundation ( CHE-0848398 ) is greatly acknowledged. Funding information: This work was supported by the National Institutes of Health (GM084396 to T.A.C. and GM065334 to D.F.) and the National Science Foundation (CHE-0848398 to T.A.C.).

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures.

AB - Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures.

KW - Aminoacyl-tRNA synthetase

KW - Ubiquitin

KW - Unnatural amino acid

KW - tRNA

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 19

ER -

Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids

Abstract

Keywords

ASJC Scopus subject areas

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