Computer assisted microdentsitometric analysis of footprinting autoradiographic data

James C. Dabrowiak; Andrew Skorobogaty; Ned Rich; Calvin P.H. Vary; John N. Vournakis

doi:10.1093/nar/14.1.489

Computer assisted microdentsitometric analysis of footprinting autoradiographic data

James C. Dabrowiak, Andrew Skorobogaty, Ned Rich, Calvin P.H. Vary, John N. Vournakis

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A system comprised of a linear scanning microdensitometer interfaced to a personal computer has been developed to facilitate analysis of ligand-DNA footprinting autoradiograms.^† The system, which can be used to record density and sequence information from autoradiographic films, enables the user to relate the area under an autoradiographic band to the concentration of radiolabeled molecules present in the electrophoresis gel. This report describes the computer program which performs the calculations and discusses the ability of the system to accurately determine oligonucleotide concentration, as a function of band separation, photographic response, and the computational algorithm used to calculate band areas.

Original language	English (US)
Pages (from-to)	489-499
Number of pages	11
Journal	Nucleic acids research
Volume	14
Issue number	1
DOIs	https://doi.org/10.1093/nar/14.1.489
State	Published - Jan 10 1986

ASJC Scopus subject areas

Genetics

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title = "Computer assisted microdentsitometric analysis of footprinting autoradiographic data",

abstract = "A system comprised of a linear scanning microdensitometer interfaced to a personal computer has been developed to facilitate analysis of ligand-DNA footprinting autoradiograms.† The system, which can be used to record density and sequence information from autoradiographic films, enables the user to relate the area under an autoradiographic band to the concentration of radiolabeled molecules present in the electrophoresis gel. This report describes the computer program which performs the calculations and discusses the ability of the system to accurately determine oligonucleotide concentration, as a function of band separation, photographic response, and the computational algorithm used to calculate band areas.",

author = "Dabrowiak, {James C.} and Andrew Skorobogaty and Ned Rich and Vary, {Calvin P.H.} and Vournakis, {John N.}",

note = "Funding Information: ACKNOWLEDGMENTS We are grateful to Drs. B. Ware and L. Plank for helpful discussions and to E. L. Fish, R. Rehfuss, M. J. Lane and L. Vriesinga for assistance and advice throughout the course of this work. The work described herein was supported by a grant from the National Institutes of Health, GM31895. One of us (J. C. D.) is grateful to the American Cancer Society for a Scholar in Cancer Research Award for 1985.",

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AU - Vournakis, John N.

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N2 - A system comprised of a linear scanning microdensitometer interfaced to a personal computer has been developed to facilitate analysis of ligand-DNA footprinting autoradiograms.† The system, which can be used to record density and sequence information from autoradiographic films, enables the user to relate the area under an autoradiographic band to the concentration of radiolabeled molecules present in the electrophoresis gel. This report describes the computer program which performs the calculations and discusses the ability of the system to accurately determine oligonucleotide concentration, as a function of band separation, photographic response, and the computational algorithm used to calculate band areas.

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Computer assisted microdentsitometric analysis of footprinting autoradiographic data

Abstract

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