Computer assisted microdentsitometric analysis of footprinting autoradiographic data

James C. Dabrowiak, Andrew Skorobogaty, Ned Rich, Calvin P.H. Vary, John N. Vournakis

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

A system comprised of a linear scanning microdensitometer interfaced to a personal computer has been developed to facilitate analysis of ligand-DNA footprinting autoradiograms. The system, which can be used to record density and sequence information from autoradiographic films, enables the user to relate the area under an autoradiographic band to the concentration of radiolabeled molecules present in the electrophoresis gel. This report describes the computer program which performs the calculations and discusses the ability of the system to accurately determine oligonucleotide concentration, as a function of band separation, photographic response, and the computational algorithm used to calculate band areas.

Original languageEnglish (US)
Pages (from-to)489-499
Number of pages11
JournalNucleic Acids Research
Volume14
Issue number1
DOIs
StatePublished - Jan 10 1986

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ASJC Scopus subject areas

  • Genetics

Cite this

Dabrowiak, J. C., Skorobogaty, A., Rich, N., Vary, C. P. H., & Vournakis, J. N. (1986). Computer assisted microdentsitometric analysis of footprinting autoradiographic data. Nucleic Acids Research, 14(1), 489-499. https://doi.org/10.1093/nar/14.1.489