TY - JOUR
T1 - Comparative study of shark and beef brain microtubules
AU - Langford, George M.
N1 - Funding Information:
I wish to gratefully acknowledge the assis. tance of Lascelles Lyn~?ook, whose help was invaluable to this project. I also wish to thank Sue Wilburn for typing the manuscript. Supported by NIH Grant GM 28107.
PY - 1981
Y1 - 1981
N2 - Shark and beef brain tubulins were prepared by a common temperature-dependent centrifugation procedure and compared on the basis of assembly properties. The data show that: (a) the conditions for spontaneous assembly without microtubule associated proteins; (b) initial rates of assembly between 20°C and 37°C; and (c) types of polymers formed in the presence of Ca2+ are different for the two types of tubulins. The number of protofilaments, on the other hand, is the same for microtubules reconstituted from the two species of tubulins. These data are interpreted to suggest that tubulins from different species have unique properties, but the tubulin sites affecting the structure of microtubules have been highly conserved.
AB - Shark and beef brain tubulins were prepared by a common temperature-dependent centrifugation procedure and compared on the basis of assembly properties. The data show that: (a) the conditions for spontaneous assembly without microtubule associated proteins; (b) initial rates of assembly between 20°C and 37°C; and (c) types of polymers formed in the presence of Ca2+ are different for the two types of tubulins. The number of protofilaments, on the other hand, is the same for microtubules reconstituted from the two species of tubulins. These data are interpreted to suggest that tubulins from different species have unique properties, but the tubulin sites affecting the structure of microtubules have been highly conserved.
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U2 - 10.1016/0303-2647(81)90032-0
DO - 10.1016/0303-2647(81)90032-0
M3 - Article
C2 - 7337808
AN - SCOPUS:0019803252
SN - 0303-2647
VL - 14
SP - 247
EP - 259
JO - BioSystems
JF - BioSystems
IS - 3-4
ER -