Combined use of 16S ribosomal DNA and automated ribosomal intergenic spacer analysis to study the bacterial community in catfish ponds

C. R. Arias, J. W. Abernathy, Z. Liu

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Aims: To apply culture-independent techniques to explore the bacterial community composition in catfish pond water. Methods and Results: 16S rDNA libraries were constructed and sequenced from 15 pond water samples. Automated ribosomal intergenic spacer analysis (ARISA) was used to fingerprint each bacterial community. A broad diversity in bacterial species composition was found by 16S rDNA analysis. Alphaproteobacteria was the most represented class in all ponds, followed by Gammaproteobacteria and Gram-positive high G + C content bacteria. Uniqueness of bacterial communities from each individual pond was confirmed by ARISA. Catfish pathogens were detected sporadically. Conclusions: Bacterial communities in a catfish aquaculture setting can vary from pond to pond at one given point. No correlation could be made between bacteria composition and fish strain or between bacterial profile and the presence of catfish pathogens in a particular pond. Significance and Impact of the Study: This is the first report showing the composition of bacterial communities in catfish ponds. Fish health specialists and catfish aquaculture managers should be aware of the wide differences in bacterial communities between ponds and include this variable in fish husbandry practices.

Original languageEnglish (US)
Pages (from-to)287-292
Number of pages6
JournalLetters in Applied Microbiology
Volume43
Issue number3
DOIs
StatePublished - Sep 1 2006
Externally publishedYes

Keywords

  • 16S rDNA
  • ARISA
  • Aquaculture
  • Bacterial communities
  • Catfish

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Combined use of 16S ribosomal DNA and automated ribosomal intergenic spacer analysis to study the bacterial community in catfish ponds'. Together they form a unique fingerprint.

  • Cite this