Combinatorial approaches to evaluate nanodiamond uptake and induced cellular fate

Reem Eldawud, Manuela Reitzig, Jörg Opitz, Yon Rojansakul, Wenjuan Jiang, Shikha Nangia, Cerasela Zoica Dinu

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Nanodiamonds (NDs) are an emerging class of engineered nanomaterials that hold great promise for the next generation of bionanotechnological products to be used for drug and gene delivery, or for bio-imaging and biosensing. Previous studies have shown that upon their cellular uptake, NDs exhibit high biocompatibility in various in vitro and in vivo set-ups. Herein we hypothesized that the increased NDs biocompatibility is a result of minimum membrane perturbations and their reduced ability to induce disruption or damage during cellular translocation. Using multi-scale combinatorial approaches that simulate ND-membrane interactions, we correlated NDs real-time cellular uptake and kinetics with the ND-induced membrane fluctuations to derive energy requirements for the uptake to occur. Our discrete and real-time analyses showed that the majority of NDs internalization occurs within 2 h of cellular exposure, however, with no effects on cellular viability, proliferation or cellular behavior. Furthermore, our simulation analyses using coarse-grained models identified key changes in the energy profile, membrane deformation and recovery time, all functions of the average ND or ND-based agglomerate size. Understanding the mechanisms responsible for ND-cell membrane interactions could possibly advance their implementation in various biomedical applications.

Original languageEnglish (US)
Article number085107
JournalNanotechnology
Volume27
Issue number8
DOIs
StatePublished - Jan 28 2016

Keywords

  • cell-based sensing
  • course grained simulation
  • nanodiamonds

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Fingerprint Dive into the research topics of 'Combinatorial approaches to evaluate nanodiamond uptake and induced cellular fate'. Together they form a unique fingerprint.

  • Cite this

    Eldawud, R., Reitzig, M., Opitz, J., Rojansakul, Y., Jiang, W., Nangia, S., & Dinu, C. Z. (2016). Combinatorial approaches to evaluate nanodiamond uptake and induced cellular fate. Nanotechnology, 27(8), [085107]. https://doi.org/10.1088/0957-4484/27/8/085107