TY - GEN
T1 - Classification of cell types using mechanical and electrical measurement on single cells
AU - Chen, J.
AU - Zheng, Y.
AU - Tan, Q.
AU - Shojaei-Baghini, E.
AU - Zhang, Y.
AU - Prasad, P.
AU - Wu, X. Y.
AU - Sun, Y.
PY - 2011
Y1 - 2011
N2 - This paper presents a microsystem for single-cell characterization by aspirating cells continuously through a constriction channel while cell impedance profiles and images are measured to quantify transit time, the impedance amplitude ratio, and cell elongation simultaneously. The system demonstrated that osteoblasts, compared with osteocytes, have a larger cell elongation length, longer transit time, and a higher amplitude ratio. The system also classified EMT6 and EMT6/AR1.0 cells with success rates of 51.3% (cell elongation), 57.5% (transit time), 59.6% (amplitude ratio), and 70.2% (both) using neural network, further verifying the device's capability for performing both electrical and mechanical measurements on single cells.
AB - This paper presents a microsystem for single-cell characterization by aspirating cells continuously through a constriction channel while cell impedance profiles and images are measured to quantify transit time, the impedance amplitude ratio, and cell elongation simultaneously. The system demonstrated that osteoblasts, compared with osteocytes, have a larger cell elongation length, longer transit time, and a higher amplitude ratio. The system also classified EMT6 and EMT6/AR1.0 cells with success rates of 51.3% (cell elongation), 57.5% (transit time), 59.6% (amplitude ratio), and 70.2% (both) using neural network, further verifying the device's capability for performing both electrical and mechanical measurements on single cells.
KW - Cellular biophysics
KW - Constriction channel
KW - Impedance spectroscopy
KW - Microfluidics
KW - Neural network
KW - Single cell analysis
UR - http://www.scopus.com/inward/record.url?scp=84883775225&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883775225&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883775225
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 795
EP - 797
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -