Circular dichroism of double-helical oligoribonucleotides

Philip N. Borer, Olke C. Uhlenbeck, Barbara Dengler, Ignacio Tinoco

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The ultraviolet circular dichroism and absorption of 15 double-stranded helical oligoribonucleotides have been measured. These molecules of chain-length 6 to 12 contain all 10 possible nearest neighbors of Watson-Crick base pairs. They are thus good models for short double-stranded regions in RNA molecules. The contribution to the circular dichroism of each of the nearest neighbor base pairs has been obtained. The circular dichroism is found to be very sequence-dependent and may be useful in distinguishing possible secondary structures. However, the nearest neighbor approximation for circular dichroism fails to give a quantitative measure of the circular dichroism of double-strand regions.

Original languageEnglish (US)
Pages (from-to)759-771
Number of pages13
JournalJournal of Molecular Biology
Volume80
Issue number4
DOIs
StatePublished - Nov 15 1973

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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    Borer, P. N., Uhlenbeck, O. C., Dengler, B., & Tinoco, I. (1973). Circular dichroism of double-helical oligoribonucleotides. Journal of Molecular Biology, 80(4), 759-771. https://doi.org/10.1016/0022-2836(73)90208-8