Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex

Catharina Hiort; Jerry Goodisman; James C. Dabrowiak

doi:10.1007/BF01075916

Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex

Catharina Hiort, Jerry Goodisman, James C. Dabrowiak

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Chemically and photochemically induced cleavage of DNA by the insulin-mimetic compound NH₄[VO(O₂)₂-(1,10-phenanthroline)], bpV(phen), have been studied.⁵¹V NMR and absorption indicate that photoirradiation with low energy UV light of aqueous solutions containing bpV(phen) leads to the conversion of the compound to simple vanadates. Photoillumination of the compound in the presence of supercoiled pBR322 DNA results in cutting of the plasmid to produce nicked circular and linear DNA. Quantitative analysis of agarose gel data shows that bpV(phen) is a single strand nicking agent exhibiting sequence and/or base specificity.

Original language	English (US)
Pages (from-to)	31-36
Number of pages	6
Journal	Molecular and Cellular Biochemistry
Volume	153
Issue number	1-2
DOIs	https://doi.org/10.1007/BF01075916
State	Published - Dec 1995

Keywords

DNA cleavage
bisperoxovanadium
insulin-mimetic
photocleavage
photolysis

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1007/BF01075916

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title = "Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex",

abstract = "Chemically and photochemically induced cleavage of DNA by the insulin-mimetic compound NH4[VO(O2)2-(1,10-phenanthroline)], bpV(phen), have been studied.51V NMR and absorption indicate that photoirradiation with low energy UV light of aqueous solutions containing bpV(phen) leads to the conversion of the compound to simple vanadates. Photoillumination of the compound in the presence of supercoiled pBR322 DNA results in cutting of the plasmid to produce nicked circular and linear DNA. Quantitative analysis of agarose gel data shows that bpV(phen) is a single strand nicking agent exhibiting sequence and/or base specificity.",

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AU - Hiort, Catharina

AU - Goodisman, Jerry

AU - Dabrowiak, James C.

PY - 1995/12

Y1 - 1995/12

N2 - Chemically and photochemically induced cleavage of DNA by the insulin-mimetic compound NH4[VO(O2)2-(1,10-phenanthroline)], bpV(phen), have been studied.51V NMR and absorption indicate that photoirradiation with low energy UV light of aqueous solutions containing bpV(phen) leads to the conversion of the compound to simple vanadates. Photoillumination of the compound in the presence of supercoiled pBR322 DNA results in cutting of the plasmid to produce nicked circular and linear DNA. Quantitative analysis of agarose gel data shows that bpV(phen) is a single strand nicking agent exhibiting sequence and/or base specificity.

AB - Chemically and photochemically induced cleavage of DNA by the insulin-mimetic compound NH4[VO(O2)2-(1,10-phenanthroline)], bpV(phen), have been studied.51V NMR and absorption indicate that photoirradiation with low energy UV light of aqueous solutions containing bpV(phen) leads to the conversion of the compound to simple vanadates. Photoillumination of the compound in the presence of supercoiled pBR322 DNA results in cutting of the plasmid to produce nicked circular and linear DNA. Quantitative analysis of agarose gel data shows that bpV(phen) is a single strand nicking agent exhibiting sequence and/or base specificity.

KW - DNA cleavage

KW - bisperoxovanadium

KW - insulin-mimetic

KW - photocleavage

KW - photolysis

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Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex

Abstract

Keywords

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