Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex

Catharina Hiort, Jerry Goodisman, James C. Dabrowiak

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Chemically and photochemically induced cleavage of DNA by the insulin-mimetic compound NH4[VO(O2)2-(1,10-phenanthroline)], bpV(phen), have been studied.51V NMR and absorption indicate that photoirradiation with low energy UV light of aqueous solutions containing bpV(phen) leads to the conversion of the compound to simple vanadates. Photoillumination of the compound in the presence of supercoiled pBR322 DNA results in cutting of the plasmid to produce nicked circular and linear DNA. Quantitative analysis of agarose gel data shows that bpV(phen) is a single strand nicking agent exhibiting sequence and/or base specificity.

Original languageEnglish (US)
Pages (from-to)31-36
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume153
Issue number1-2
DOIs
StatePublished - Dec 1995

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Keywords

  • bisperoxovanadium
  • DNA cleavage
  • insulin-mimetic
  • photocleavage
  • photolysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

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