Characterization of the gene for the Fe-protein of the vanadium dependent alternative nitrogenase of Azotobacter vinelandii and construction of a Tn 5 mutant

Ramesh Raina, M. Amarender Reddy, Debabrota Ghosal, H. K. Das

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A sequence homologous to the conventional nifH gene has been cloned from a different region of the Azotobacter vinelandii genome. Tn 5 insertions were obtained in this clone and the mutagenized plasmid was used for marker exchange with A. vinelandii strain CA12 (ΔnifHDK) to obtain Tn 5 mutants. These mutants exhibited a Nif- phenotype in the presence of vanadium, unlike CA12 which was Nif+ on vanadium-containing medium. The gene in the cloned nifH-like region is therefore apparently involved in the vanadium dependent alternative pathway of nitrogen fixation. This gene, nifH2, has been sequenced and encodes a protein of 289 amino acids that is similar to nifH in nucleotide sequence, deduced amino acid sequence, predicted secondary structure and hydrophobicity profile. A second open reading frame downstream of nifH2 codes for a protein of 64 amino acids, similar to the ferredoxin (Fd)-like protein encoded downstream of nifH* in A. chroococcum. Sequence analysis suggests that the nifH2 and Fd-like genes are in a single operon.

Original languageEnglish (US)
Pages (from-to)121-127
Number of pages7
JournalMGG Molecular & General Genetics
Volume214
Issue number1
DOIs
StatePublished - Sep 1988
Externally publishedYes

Fingerprint

Azotobacter vinelandii
Nitrogenase
Vanadium
Ferredoxins
Genes
Proteins
Amino Acids
Nitrogen Fixation
Operon
Sequence Homology
Hydrophobic and Hydrophilic Interactions
Open Reading Frames
Sequence Analysis
Amino Acid Sequence
Plasmids
Clone Cells
Genome
Phenotype

Keywords

  • Azotobacter vinelandii
  • DNA sequencing
  • Marker exchange
  • nifH2
  • Vanadium nitrogenase

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of the gene for the Fe-protein of the vanadium dependent alternative nitrogenase of Azotobacter vinelandii and construction of a Tn 5 mutant. / Raina, Ramesh; Reddy, M. Amarender; Ghosal, Debabrota; Das, H. K.

In: MGG Molecular & General Genetics, Vol. 214, No. 1, 09.1988, p. 121-127.

Research output: Contribution to journalArticle

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