TY - JOUR
T1 - Characterization of a mannose-binding lectin from channel catfish (Ictalurus punctatus)
AU - Zhang, Hao
AU - Peatman, Eric
AU - Liu, Hong
AU - Niu, Donghong
AU - Feng, Tingting
AU - Kucuktas, Huseyin
AU - Waldbieser, Geoff
AU - Chen, Liqiao
AU - Liu, Zhanjiang
N1 - Funding Information:
This project was supported by a grant from the USDA AFRI Animal Genome Tools and Resources Program (USDA/NRICGP award# 2009-35205-05101) and in part by USDA, ARS CRIS 6402-31000-08-00D. The authors would like to thank Jianguo Lu and Shikai Liu for assistance in database searches.
PY - 2012/6
Y1 - 2012/6
N2 - Mannose-binding lectin (MBL) is an important component of innate immunity capable of activating the lectin pathway of the complement system. A MBL gene was isolated from channel catfish (Ictalurus punctatus). The deduced protein contains a canonical collagen-like domain, a carbohydrate recognition domain (CRD), and a neck region similar to mammalian mannose-binding lectin. The catfish mannose-binding lectin CRD contains the EPN motif shown previously to mediate mannose specificity. The catfish mannose-binding lectin showed 30-43% identity with MBL protein sequences of rainbow trout, zebrafish, common carp, and goldfish, and 33-35% identity with sequences of mammalian species. In this study, while liver was the predominant source of mannose-binding lectin gene expression in healthy tissues, mannose-binding lectin expression in spleen rose sharply following challenge with a Gram-negative bacterium.
AB - Mannose-binding lectin (MBL) is an important component of innate immunity capable of activating the lectin pathway of the complement system. A MBL gene was isolated from channel catfish (Ictalurus punctatus). The deduced protein contains a canonical collagen-like domain, a carbohydrate recognition domain (CRD), and a neck region similar to mammalian mannose-binding lectin. The catfish mannose-binding lectin CRD contains the EPN motif shown previously to mediate mannose specificity. The catfish mannose-binding lectin showed 30-43% identity with MBL protein sequences of rainbow trout, zebrafish, common carp, and goldfish, and 33-35% identity with sequences of mammalian species. In this study, while liver was the predominant source of mannose-binding lectin gene expression in healthy tissues, mannose-binding lectin expression in spleen rose sharply following challenge with a Gram-negative bacterium.
KW - Channel catfish
KW - Edwardsiella ictaluri
KW - Ictalurus punctatus
KW - Lectin
KW - MBL
KW - Mannose-binding lectin
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U2 - 10.1016/j.rvsc.2011.03.024
DO - 10.1016/j.rvsc.2011.03.024
M3 - Article
C2 - 21524427
AN - SCOPUS:84859102439
SN - 0034-5288
VL - 92
SP - 408
EP - 413
JO - Research in Veterinary Science
JF - Research in Veterinary Science
IS - 3
ER -