Characterization of a mannose-binding lectin from channel catfish (Ictalurus punctatus)

Hao Zhang, Eric Peatman, Hong Liu, Donghong Niu, Tingting Feng, Huseyin Kucuktas, Geoff Waldbieser, Liqiao Chen, Zhanjiang Liu

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Mannose-binding lectin (MBL) is an important component of innate immunity capable of activating the lectin pathway of the complement system. A MBL gene was isolated from channel catfish (Ictalurus punctatus). The deduced protein contains a canonical collagen-like domain, a carbohydrate recognition domain (CRD), and a neck region similar to mammalian mannose-binding lectin. The catfish mannose-binding lectin CRD contains the EPN motif shown previously to mediate mannose specificity. The catfish mannose-binding lectin showed 30-43% identity with MBL protein sequences of rainbow trout, zebrafish, common carp, and goldfish, and 33-35% identity with sequences of mammalian species. In this study, while liver was the predominant source of mannose-binding lectin gene expression in healthy tissues, mannose-binding lectin expression in spleen rose sharply following challenge with a Gram-negative bacterium.

Original languageEnglish (US)
Pages (from-to)408-413
Number of pages6
JournalResearch in Veterinary Science
Volume92
Issue number3
DOIs
StatePublished - Jun 2012
Externally publishedYes

Keywords

  • Channel catfish
  • Edwardsiella ictaluri
  • Ictalurus punctatus
  • Lectin
  • MBL
  • Mannose-binding lectin

ASJC Scopus subject areas

  • veterinary(all)

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