TY - JOUR
T1 - Characteristics of ionic transport processes in fish intestinal epithelial cells
AU - Movileanu, Liviu
AU - Flonta, Maria Luiza
AU - Mihailescu, Dan
AU - Frangopol, Petre T.
N1 - Funding Information:
The present work was supported by a grant of the National Council for Advanced Scientific Research of Romania (Nr. 5009/A6/1997).
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 1998/2
Y1 - 1998/2
N2 - A general mathematical version of the cell model of a leaky epithelium for the NaCl absorption is presented, analysed and integrated numerically. The model consists in the adequate differential equations that describe the rate of change of the intracellular ion concentrations and are expressed in strict accordance with the law of mass conservation. The model includes many state variables representing ion concentrations, the cell volume, and membrane potentials. Ion movements are described by the Michaelis-Menten kinetics or by the constant field flux equation. In this paper, we model the intracellular ion concentrations, change in the cell volume, the transmembrane flux and membrane potentials of intestinal epithelium of both fresh water and sea water fish, and generate several simulations (in both the steady state and the transient state analysis) that appear to accord with prior experimental data in this area. For the ion movements of the sea water fish intestine, there were included a Na+/K+ pump, a K+-Cl- symport system, the K+ and Cl- channels in the basolateral membrane, whereas a Na+-K+-2Cl- cotransporter for NaCl absorption and K+ channels are located in the apical membrane. In the fresh water fish intestinal cells, the NaCl absorption is performed by two coupled antiporters Na+/H+ and Cl-/HCO3 - presumably responsible for the intracellular pH regulation. In this type of cells, Na+ and K+ channels are located within the apical membrane, whereas Cl- channels are located within the basolateral membrane. The osmotically induced water transport across the apical and basolateral membranes has been taken into account as well. The simulations plot the steady state values for membrane potential difference, short-circuit current and intracellular ionic concentrations using the magnitude of the transmembrane flux through the Na+/K+ pump and Na+-K+-2Cl- cotransporter, or the basolateral Cl- permeability as dependent variables. The model behaves appropriately with regard to several experimental studies regarding the hyperpolarization (sea water fish intestine) and depolarization (fresh water fish intestine) of the apical membrane potential and inhibition of the short-circuit flux with reduced NaCl absorption. The model is also used to make several analytical predictions regarding the response of the membrane potential and ionic concentrations to variations in the basolateral Cl- flux. Furthermore, maintaining conservation of both mass and electroneutrality and taking into account the osmolar forces is an important advantage, because it allows a rigorous analysis of the relationship between membrane potential difference, volume and flux. The model can be used in the analysis and planning of the experiments and is capable of predicting the instantaneous values of ionic fluxes and intracellular concentrations and of cell volume.
AB - A general mathematical version of the cell model of a leaky epithelium for the NaCl absorption is presented, analysed and integrated numerically. The model consists in the adequate differential equations that describe the rate of change of the intracellular ion concentrations and are expressed in strict accordance with the law of mass conservation. The model includes many state variables representing ion concentrations, the cell volume, and membrane potentials. Ion movements are described by the Michaelis-Menten kinetics or by the constant field flux equation. In this paper, we model the intracellular ion concentrations, change in the cell volume, the transmembrane flux and membrane potentials of intestinal epithelium of both fresh water and sea water fish, and generate several simulations (in both the steady state and the transient state analysis) that appear to accord with prior experimental data in this area. For the ion movements of the sea water fish intestine, there were included a Na+/K+ pump, a K+-Cl- symport system, the K+ and Cl- channels in the basolateral membrane, whereas a Na+-K+-2Cl- cotransporter for NaCl absorption and K+ channels are located in the apical membrane. In the fresh water fish intestinal cells, the NaCl absorption is performed by two coupled antiporters Na+/H+ and Cl-/HCO3 - presumably responsible for the intracellular pH regulation. In this type of cells, Na+ and K+ channels are located within the apical membrane, whereas Cl- channels are located within the basolateral membrane. The osmotically induced water transport across the apical and basolateral membranes has been taken into account as well. The simulations plot the steady state values for membrane potential difference, short-circuit current and intracellular ionic concentrations using the magnitude of the transmembrane flux through the Na+/K+ pump and Na+-K+-2Cl- cotransporter, or the basolateral Cl- permeability as dependent variables. The model behaves appropriately with regard to several experimental studies regarding the hyperpolarization (sea water fish intestine) and depolarization (fresh water fish intestine) of the apical membrane potential and inhibition of the short-circuit flux with reduced NaCl absorption. The model is also used to make several analytical predictions regarding the response of the membrane potential and ionic concentrations to variations in the basolateral Cl- flux. Furthermore, maintaining conservation of both mass and electroneutrality and taking into account the osmolar forces is an important advantage, because it allows a rigorous analysis of the relationship between membrane potential difference, volume and flux. The model can be used in the analysis and planning of the experiments and is capable of predicting the instantaneous values of ionic fluxes and intracellular concentrations and of cell volume.
KW - Computer simulation
KW - Fish intestinal cells
KW - Ionic transport mechanisms
KW - The NaCl absorption model
KW - The steady-state analysis
UR - http://www.scopus.com/inward/record.url?scp=0031909794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031909794&partnerID=8YFLogxK
U2 - 10.1016/S0303-2647(97)00071-3
DO - 10.1016/S0303-2647(97)00071-3
M3 - Article
C2 - 9544403
AN - SCOPUS:0031909794
VL - 45
SP - 123
EP - 140
JO - Currents in modern biology
JF - Currents in modern biology
SN - 0303-2647
IS - 2
ER -