Channel catfish hemoglobin genes: Identification, phylogenetic and syntenic analysis, and specific induction in response to heat stress

Jianbin Feng, Shikai Liu, Xiuli Wang, Ruijia Wang, Jiaren Zhang, Yanliang Jiang, Chao Li, Ludmilla Kaltenboeck, Jiale Li, Zhanjiang Liu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Hemoglobins transport oxygen from gill to inner organs in fish, and this process is affected by temperature, one of the major environmental factors for fish. The hemoglobin gene clusters have been well studied in humans and several model fish species, but remain largely unknown in catfish. Here, eight α- and six β-hemoglobin genes were identified and characterized in channel catfish. Genomic synteny analysis showed that these hemoglobin genes were separated into two unlinked clusters, the MN cluster containing six α- and six β-hemoglobin genes, and the LA cluster consisting of two α-hemoglobin genes. Channel catfish hemoglobin genes were ubiquitously expressed in all the 10 tested tissues from healthy fish, but exhibited higher expression level in spleen, head kidney, and trunk kidney. In response to heat stress, hemoglobin genes, especially MN Hbα4, MN Hbα5, MN Hbα6, MN Hbβ4, MN Hbβ5, MN Hbβ6, LA Hbα1, and LA Hbα2, presumably the embryonic hemoglobin genes, were drastically up-regulated in the gill and head kidney of heat-tolerant fishes, but not in these tissues of the heat-intolerant fish, suggesting the importance of the embryonic hemoglobin genes in coping with the low oxygen conditions under heat stress.

Original languageEnglish (US)
Pages (from-to)11-22
Number of pages12
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume9
Issue number1
DOIs
StatePublished - Mar 2014
Externally publishedYes

Keywords

  • Catfish
  • Expression
  • Heat stress
  • Hemoglobin
  • Synteny

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Channel catfish hemoglobin genes: Identification, phylogenetic and syntenic analysis, and specific induction in response to heat stress'. Together they form a unique fingerprint.

Cite this