Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle

Agnik Dasgupta, Matthias Merkel, Madeline J. Clark, Andrew E. Jacob, Jonathan Edward Dawson, Mary Elizabeth Manning, Jeffrey D. Amack

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

How epithelial cell behaviors are coordinately regulated to sculpt tissue architecture is a fundamental question in biology. Kupffer’s vesicle (KV), a transient organ with a fluid-filled lumen, provides a simple system to investigate the interplay between intrinsic cellular mechanisms and external forces during epithelial morphogenesis. Using 3-dimensional (3D) analyses of single cells we identify asymmetric cell volume changes along the anteroposterior axis of KV that coincide with asymmetric cell shape changes. Blocking ion flux prevents these cell volume changes and cell shape changes. Vertex simulations suggest cell shape changes do not depend on lumen expansion. Consistent with this prediction, asymmetric changes in KV cell volume and shape occur normally when KV lumen growth fails due to leaky cell adhesions. These results indicate ion flux mediates cell volume changes that contribute to asymmetric cell shape changes in KV, and that these changes in epithelial morphology are separable from lumen-generated forces.

Original languageEnglish (US)
Article numbere30963
JournaleLife
Volume7
DOIs
StatePublished - Jan 29 2018

    Fingerprint

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Dasgupta, A., Merkel, M., Clark, M. J., Jacob, A. E., Dawson, J. E., Manning, M. E., & Amack, J. D. (2018). Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle. eLife, 7, [e30963]. https://doi.org/10.7554/eLife.30963