@article{752dd55aedba4c8d9c9cc967ffa0968a,
title = "Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer{\textquoteright}s vesicle",
abstract = "How epithelial cell behaviors are coordinately regulated to sculpt tissue architecture is a fundamental question in biology. Kupffer{\textquoteright}s vesicle (KV), a transient organ with a fluid-filled lumen, provides a simple system to investigate the interplay between intrinsic cellular mechanisms and external forces during epithelial morphogenesis. Using 3-dimensional (3D) analyses of single cells we identify asymmetric cell volume changes along the anteroposterior axis of KV that coincide with asymmetric cell shape changes. Blocking ion flux prevents these cell volume changes and cell shape changes. Vertex simulations suggest cell shape changes do not depend on lumen expansion. Consistent with this prediction, asymmetric changes in KV cell volume and shape occur normally when KV lumen growth fails due to leaky cell adhesions. These results indicate ion flux mediates cell volume changes that contribute to asymmetric cell shape changes in KV, and that these changes in epithelial morphology are separable from lumen-generated forces.",
author = "Agnik Dasgupta and Matthias Merkel and Clark, {Madeline J.} and Jacob, {Andrew E.} and Dawson, {Jonathan Edward} and Manning, {M. Lisa} and Amack, {Jeffrey D.}",
note = "Funding Information: Simons Foundation#446222 Matthias Merkel M Lisa ManningResearch Corporation for Scientific Advancement Cottrell Scholar program Matthias Merkel M Lisa Manning Gordon and Betty Moore Foundation Matthias Merkel M Lisa ManningNational Institutes of Health R01GM117598 M Lisa ManningNational Institutes of Health R01HL095690 Jeffrey D Amack Funding Information: We thank Jeffrey Saffitz, Stephanie Woo and Maura Grealy for sharing reagents. We also thank members of the Amack and Manning groups for helpful discussions and Gonca Erdemci-Tandogan for providing critical feedback on the manuscript. A special thank you to Fiona Foley and Sharleen Buel for outstanding technical support. This work was supported by NIH grants R01HL095690 (JDA) and R01GM117598 (MLM). Additional support was provided by a grant from the Simons Foundation (#446222 MLM and MM), the Research Corporation for Scientific Advancement through the Cottrell Scholars Program (MLM and MM), and through the Gordon and Betty Moore Foundation (MLM and MM). Computing infrastructure support was provided through NSF ACI-1541396. Publisher Copyright: {\textcopyright} Dasgupta et al.",
year = "2018",
month = jan,
day = "29",
doi = "10.7554/eLife.30963",
language = "English (US)",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}