Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle

Agnik Dasgupta, Matthias Merkel, Madeline J. Clark, Andrew E. Jacob, Jonathan Edward Dawson, Mary Elizabeth Manning, Jeffrey D. Amack

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

How epithelial cell behaviors are coordinately regulated to sculpt tissue architecture is a fundamental question in biology. Kupffer’s vesicle (KV), a transient organ with a fluid-filled lumen, provides a simple system to investigate the interplay between intrinsic cellular mechanisms and external forces during epithelial morphogenesis. Using 3-dimensional (3D) analyses of single cells we identify asymmetric cell volume changes along the anteroposterior axis of KV that coincide with asymmetric cell shape changes. Blocking ion flux prevents these cell volume changes and cell shape changes. Vertex simulations suggest cell shape changes do not depend on lumen expansion. Consistent with this prediction, asymmetric changes in KV cell volume and shape occur normally when KV lumen growth fails due to leaky cell adhesions. These results indicate ion flux mediates cell volume changes that contribute to asymmetric cell shape changes in KV, and that these changes in epithelial morphology are separable from lumen-generated forces.

Original languageEnglish (US)
Article numbere30963
JournaleLife
Volume7
DOIs
StatePublished - Jan 29 2018

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Cell Shape
Zebrafish
Morphogenesis
Cell Size
Ions
Fluxes
Cell adhesion
Tissue
Fluids
Single-Cell Analysis
Cell Adhesion
Epithelial Cells
Growth

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Dasgupta, A., Merkel, M., Clark, M. J., Jacob, A. E., Dawson, J. E., Manning, M. E., & Amack, J. D. (2018). Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle. eLife, 7, [e30963]. https://doi.org/10.7554/eLife.30963

Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle. / Dasgupta, Agnik; Merkel, Matthias; Clark, Madeline J.; Jacob, Andrew E.; Dawson, Jonathan Edward; Manning, Mary Elizabeth; Amack, Jeffrey D.

In: eLife, Vol. 7, e30963, 29.01.2018.

Research output: Contribution to journalArticle

Dasgupta, A, Merkel, M, Clark, MJ, Jacob, AE, Dawson, JE, Manning, ME & Amack, JD 2018, 'Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle', eLife, vol. 7, e30963. https://doi.org/10.7554/eLife.30963
Dasgupta, Agnik ; Merkel, Matthias ; Clark, Madeline J. ; Jacob, Andrew E. ; Dawson, Jonathan Edward ; Manning, Mary Elizabeth ; Amack, Jeffrey D. / Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle. In: eLife. 2018 ; Vol. 7.
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