Cell microencapsulation in polyethylene glycol hydrogel microspheres using electrohydrodynamic spraying

Mozhdeh Imaninezhad, Era Jain, Silviya Petrova Zustiak

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Microencapsulation of cells is beneficial for various biomedical applications, such as tissue regeneration and cell delivery. While a variety of techniques can be used to produce microspheres, electrohydrodynamic spraying (EHS) has shown promising results for the fabrication of cell-laden hydrogel microspheres in a wide range of sizes and in a relatively high-throughput manner. Here we describe an EHS technique for the fabrication of cell-laden polyethylene glycol (PEG) microspheres. We utilize mild hydrogel gelation chemistry and a combination of EHS parameters to allow for cell microencapsulation with high efficiency and viability. We also give examples on the effect of different EHS parameters such as inner diameter of the needle, voltage and flow rate on microsphere size and encapsulated cell viability.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages313-325
Number of pages13
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1576
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell microencapsulation
  • Electrospraying
  • Hydrogels
  • Microspheres
  • Polyethylene glycol (PEG)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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