@article{c12bfa1b2f5b41f09eb8f2690e8d0b4d,
title = "Caveolin1 identifies a specific subpopulation of cerebral cortex callosal projection neurons (CPN) including dual projecting cortical callosal/frontal projection neurons (CPN/FPN)",
abstract = "The neocortex is composed of many distinct subtypes of neurons that must form precise subtype-specific connections to enable the cortex to perform complex functions. Callosal projection neurons (CPN) are the broad population of commissural neurons that connect the cerebral hemispheres via the corpus callosum (CC). Currently, how the remarkable diversity of CPN subtypes and connectivity is specified, and how they differentiate to form highly precise and specific circuits, are largely unknown. We identify in mouse that the lipid-bound scaffolding domain protein Caveolin 1 (CAV1) is specifically expressed by a unique subpopulation of Layer V CPN that maintain dual ipsilateral frontal projections to premotor cortex. CAV1 is expressed by over 80% of these dual projecting callosal/frontal projection neurons (CPN/FPN), wiThexpression peaking early postnatally as axonal and dendritic targets are being reached and refined. CAV1 is localized to the soma and dendrites of CPN/FPN, a unique population of neurons that shares information both between hemispheres and with premotor cortex, suggesting function during postmitotic development and refinement of these neurons, rather than in their specification. Consistent with this, we find that Cav1 function is not necessary for the early specification of CPN/FPN, or for projecting to their dual axonal targets. CPN subtype-specific expression of Cav1 identifies and characterizes a first molecular component that distinguishes this functionally unique projection neuron population, a population that expands in primates, and is prototypical of additional dual and higher-order projection neuron subtypes.",
keywords = "Axonal projections, Connectivity, Corpus callosum, Neocortex, Neuronal subtypes",
author = "MacDonald, {Jessica L.} and Fame, {Ryann M.} and Gillis-Buck, {Eva M.} and Macklis, {Jeffrey D.}",
note = "Funding Information: This work was supported by grants from the National Institutes of Health (R37 NS41590, with additional infrastructure support by NS45523, NS49553, and NS075672), the Jane and Lee Seidman Fund, the Emily and Robert Pearlstein Fund, and the United Sydney Association (to J.D.M); and by the National Science Foundation Graduate Research Fellowship Program fellowship and the National Institutes of Health Predoctoral NRSA Fellowship F31 NS073163 (to R.M.F). Funding Information: Received July 5, 2017; accepted December 19, 2017; First published January 8, 2018. The authors declare no competing financial interests. Author contributions: J.L.M., R.M.F., and J.D.M. designed research; J.L.M., R.M.F., and E.M.G.-B. performed research; J.L.M. and R.M.F. analyzed data; J.L.M., R.M.F., and J.D.M. wrote the paper. This work was supported by grants from the National Institutes of Health (R37 NS41590, with additional infrastructure support by NS45523, NS49553, and NS075672), the Jane and Lee Seidman Fund, the Emily and Robert Pearlstein Fund, and the United Sydney Association (to J.D.M); and by the National Science Foundation Graduate Research Fellowship Program fellowship and the National Institutes of Health Predoctoral NRSA Fellowship F31 NS073163 (to R.M.F). § J.L.M. and R.M.F. contributed equally to this work. J. L. MacDonald{\textquoteright}s present address: Department of Biology, Syracuse University, Syracuse, NY 13244. Acknowledgements: We thank members of the Macklis lab for thoughtful discussions and input, in particular G. Cederquist for sharing expertise regarding CPN/BPN; and L. Pasquina, R. Richardson, C. Greppi, T. Keefe, and P. Davis for superb technical assistance. *Correspondence should be addressed to either of the following: Jessica L. MacDonald at the above address, E-mail: jemacdon@syr.edu; Jeffrey D. Macklis at the above address, E-mail: jeffrey_macklis@harvard.edu. DOI:http://dx.doi.org/10.1523/ENEURO.0234-17.2017 Copyright {\textcopyright} 2018 MacDonald et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Publisher Copyright: {\textcopyright} 2018 MacDonald et al.",
year = "2018",
month = jan,
day = "1",
doi = "10.1523/ENEURO.0234-17.2017",
language = "English (US)",
volume = "5",
journal = "eNeuro",
issn = "2373-2822",
publisher = "Society for Neuroscience",
number = "1",
}