Cation selectivity is a conserved feature in the OccD subfamily of Pseudomonas aeruginosa

Jiaming Liu, Aaron J. Wolfe, Elif Eren, Jagamya Vijayaraghavan, Mridhu Indic, Bert Van Den Berg, Liviu Movileanu

Research output: Contribution to journalArticle

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Abstract

To achieve the uptake of small, water-soluble nutrients, Pseudomonas aeruginosa, a pathogenic Gram-negative bacterium, employs substrate-specific channels located within its outer membrane. In this paper, we present a detailed description of the single-channel characteristics of six members of the outer membrane carboxylate channel D (OccD) subfamily. Recent structural studies showed that the OccD proteins share common features, such as a closely related, monomeric, 18-stranded β-barrel conformation and large extracellular loops, which are folded back into the channel lumen. Here, we report that the OccD proteins displayed single-channel activity with a unitary conductance covering an unusually broad range, between 20 and 670 pS, as well as a diverse gating dynamics. Interestingly, we found that cation selectivity is a conserved trait among all members of the OccD subfamily, bringing a new distinction between the members of the OccD subfamily and the anion-selective OccK channels. Conserved cation selectivity of the OccD channels is in accord with an increased specificity and selectivity of these proteins for positively charged, carboxylate-containing substrates.

Original languageEnglish (US)
Pages (from-to)2908-2916
Number of pages9
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1818
Issue number11
DOIs
StatePublished - Nov 2012

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Ion Channels
Pseudomonas aeruginosa
Cations
Bronchiolo-Alveolar Adenocarcinoma
Proteins
Substrates
Gram-Negative Bacteria
Anions
Food
Membranes
Water
Bufo arenarum
Birth Injuries
Spontaneous Fractures

Keywords

  • Single-channel electrical recording
  • Single-molecule biophysics
  • Spontaneous gating
  • The OccD subfamily
  • The OprD family

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Liu, J., Wolfe, A. J., Eren, E., Vijayaraghavan, J., Indic, M., Van Den Berg, B., & Movileanu, L. (2012). Cation selectivity is a conserved feature in the OccD subfamily of Pseudomonas aeruginosa. Biochimica et Biophysica Acta - Biomembranes, 1818(11), 2908-2916. DOI: 10.1016/j.bbamem.2012.07.009

Cation selectivity is a conserved feature in the OccD subfamily of Pseudomonas aeruginosa. / Liu, Jiaming; Wolfe, Aaron J.; Eren, Elif; Vijayaraghavan, Jagamya; Indic, Mridhu; Van Den Berg, Bert; Movileanu, Liviu.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1818, No. 11, 11.2012, p. 2908-2916.

Research output: Contribution to journalArticle

Liu, J, Wolfe, AJ, Eren, E, Vijayaraghavan, J, Indic, M, Van Den Berg, B & Movileanu, L 2012, 'Cation selectivity is a conserved feature in the OccD subfamily of Pseudomonas aeruginosa' Biochimica et Biophysica Acta - Biomembranes, vol 1818, no. 11, pp. 2908-2916. DOI: 10.1016/j.bbamem.2012.07.009
Liu J, Wolfe AJ, Eren E, Vijayaraghavan J, Indic M, Van Den Berg B et al. Cation selectivity is a conserved feature in the OccD subfamily of Pseudomonas aeruginosa. Biochimica et Biophysica Acta - Biomembranes. 2012 Nov;1818(11):2908-2916. Available from, DOI: 10.1016/j.bbamem.2012.07.009

Liu, Jiaming; Wolfe, Aaron J.; Eren, Elif; Vijayaraghavan, Jagamya; Indic, Mridhu; Van Den Berg, Bert; Movileanu, Liviu / Cation selectivity is a conserved feature in the OccD subfamily of Pseudomonas aeruginosa.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1818, No. 11, 11.2012, p. 2908-2916.

Research output: Contribution to journalArticle

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