Calcineurin-dependent Protein Phosphorylation Changes During Egg Activation in Drosophila melanogaster

Zijing Zhang, Yasir H. Ahmed-Braimah, Michael L. Goldberg, Mariana F. Wolfner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In almost all animals studied to date, the crucial process of egg activation, by which an arrested mature oocyte transitions into an actively developing embryo, initiates with an increase in Ca 2 in the oocyte’s cytoplasm. This Ca 2 rise sets off a series of downstream events, including the completion of meiosis and the dynamic remodeling of the oocyte transcriptome and proteome, which prepares the oocyte for embryogenesis. Calcineurin is a highly conserved phosphatase that is activated by Ca 2 upon egg activation and that is required for the resumption of meiosis in Xenopus, ascidians, and Drosophila. The molecular mechanisms by which calcineurin transduces the calcium signal to regulate meiosis and other downstream events are still unclear. In this study, we investigate the regulatory role of calcineurin during egg activation in Drosophila melanogaster. Using mass spectrometry, we quantify the phosphoproteomic and proteomic changes that occur during egg activation, and we examine how these events are affected when calcineurin function is perturbed in female germ cells. Our results show that calcineurin regulates hundreds of phosphosites and also influences the abundance of numerous proteins during egg activation. We find calcineurin-dependent changes in cell cycle regulators including Fizzy (Fzy), Greatwall (Gwl) and Endosulfine (Endos); in protein translation modulators including PNG, NAT, eIF4G, and eIF4B; and in important components of signaling pathways including GSK3 and Akt1. Our results help elucidate the events that occur during the transition from oocyte to embryo.

Original languageEnglish (US)
Pages (from-to)S145-S158
JournalMolecular and Cellular Proteomics
Volume18
DOIs
StatePublished - Mar 2019
Externally publishedYes

Fingerprint

Phosphorylation
Calcineurin
Drosophila melanogaster
Ovum
Chemical activation
Oocytes
Meiosis
Proteins
Embryonic Structures
Cells
Egg Proteins
Urochordata
Protein Biosynthesis
Proteome
Xenopus
Transcriptome
Phosphoric Monoester Hydrolases
Germ Cells
Proteomics
Modulators

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

Calcineurin-dependent Protein Phosphorylation Changes During Egg Activation in Drosophila melanogaster. / Zhang, Zijing; Ahmed-Braimah, Yasir H.; Goldberg, Michael L.; Wolfner, Mariana F.

In: Molecular and Cellular Proteomics, Vol. 18, 03.2019, p. S145-S158.

Research output: Contribution to journalArticle

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