Cadherin-based intercellular adhesions organize epithelial cell-matrix traction forces

Aaron F. Mertz, Yonglu Che, Shiladitya Banerjee, Jill M. Goldstein, Kathryn A. Rosowski, Stephen F. Revilla, Carien M. Niessen, M. Cristina Marchetti, Eric R. Dufresne, Valerie Horsley

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

Cell-cell and cell-matrix adhesions play essential roles in the function of tissues. There is growing evidence for the importance of cross talk between these two adhesion types, yet little is known about the impact of these interactions on the mechanical coupling of cells to the extracellular matrix (ECM). Here, we combine experiment and theory to reveal how intercellular adhesions modulate forces transmitted to the ECM. In the absence of cadherin-based adhesions, primary mouse keratinocytes within a colony appear to act independently, with significant traction forces extending throughout the colony. In contrast, with strong cadherin-based adhesions, keratinocytes in a cohesive colony localize traction forces to the colony periphery. Through genetic or antibody-mediated loss of cadherin expression or function, we show that cadherin-based adhesions are essential for this mechanical cooperativity. A minimal physical model in which cell-cell adhesions modulate the physical cohesion between contractile cells is sufficient to recreate the spatial rearrangement of traction forces observed experimentally with varying strength of cadherin-based adhesions. This work defines the importance of cadherin-based cell-cell adhesions in coordinating mechanical activity of epithelial cells and has implications for the mechanical regulation of epithelial tissues during development, homeostasis, and disease.

Original languageEnglish (US)
Pages (from-to)842-847
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number3
DOIs
StatePublished - Jan 15 2013

Keywords

  • Mechanotransduction
  • Traction force microscopy

ASJC Scopus subject areas

  • General

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    Mertz, A. F., Che, Y., Banerjee, S., Goldstein, J. M., Rosowski, K. A., Revilla, S. F., Niessen, C. M., Marchetti, M. C., Dufresne, E. R., & Horsley, V. (2013). Cadherin-based intercellular adhesions organize epithelial cell-matrix traction forces. Proceedings of the National Academy of Sciences of the United States of America, 110(3), 842-847. https://doi.org/10.1073/pnas.1217279110