Abstract
The resonances of the protonated carbons of [d(TAGCGCTA)]2 have been assigned by the two-dimensional proton-detected double-quantum heteronuclear correlation experiment ([1Hl3C]-DQCOSY).13C-coupled andl3C-decoupled versions of the experiment were used. The assignment method is discussed in detail. The deoxyribose cross peaks segregate into five well-resolved regions, and the base cross peaks have distinct features that are helpful for assignments. The cross peaks from the ‘H-13C pairs at the Cyd5, Ado2 and ThdCH3 base positions fall in separate regions of the spectrum from each other; they also are resolved from the closely spaced Ado8, Guo8, Cyd6 and Thd6. Additional parameters for distinction of the base signals are their differing J-coupling values and long-range coupling patterns.
Original language | English (US) |
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Pages (from-to) | 1089-1100 |
Number of pages | 12 |
Journal | Journal of Biomolecular Structure and Dynamics |
Volume | 5 |
Issue number | 5 |
DOIs | |
State | Published - Apr 1988 |
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology