@article{b47ffc4611114a7fb3d96c3c2e320a58,
title = "Brominated Luciferins Are Versatile Bioluminescent Probes",
abstract = "We report a set of brominated luciferins for bioluminescence imaging. These regioisomeric scaffolds were accessed by using a common synthetic route. All analogues produced light with firefly luciferase, although varying levels of emission were observed. Differences in photon output were analyzed by computation and photophysical measurements. The brightest brominated luciferin was further evaluated in cell and animal models. At low doses, the analogue outperformed the native substrate in cells. The remaining luciferins, although weak emitters with firefly luciferase, were inherently capable of light production and thus potential substrates for orthogonal mutant enzymes.",
keywords = "Appel's salt, bioluminescence, imaging, luciferase, luciferin",
author = "Steinhardt, {Rachel C.} and Rathbun, {Colin M.} and Krull, {Brandon T.} and Yu, {Jason M.} and Yuhang Yang and Nguyen, {Brian D.} and Jake Kwon and McCutcheon, {David C.} and Jones, {Krysten A.} and Filipp Furche and Prescher, {Jennifer A.}",
note = "Funding Information: This work was supported by the National Institutes of Health (R01 GM107630 to J.A.P.). and the Department of Energy (DE-SC0008694 to F.F.). C.M.R. was supported by a National Science Foundation Graduate Research Fellowship (DGE-1321846). D.C.M. was supported, in part, by a fellowship from Allergan. We thank members of the Overman, Jarvo, Esser-Kahn, and Rychnovsky laboratories for providing reagents and equipment. We also thank Drs. John Greaves and Beniam Berhane for assistance with mass spectrometry analyses. Last, we acknowledge the Laser Spectroscopy Facilty at the University of California, Irvine. Publisher Copyright: {\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2017",
month = jan,
day = "3",
doi = "10.1002/cbic.201600564",
language = "English (US)",
volume = "18",
pages = "96--100",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "1",
}