TY - JOUR
T1 - Bacterial Response to Shape-Memory Actuated Silk Wrinkled Surface Topographies as a Strategy for Biofilm Prevention
AU - Oguntade, Elizabeth
AU - Owuor, Luiza
AU - Du, Changling
AU - Acierto, Anthony
AU - Meyer, Sadie
AU - Monroe, Mary Beth Browning
AU - Henderson, James H.
N1 - Publisher Copyright:
© 2024 The Author(s). Advanced Materials Interfaces published by Wiley-VCH GmbH.
PY - 2024
Y1 - 2024
N2 - Bacterial biofilms on the surfaces of indwelling biomedical devices can cause long-term infection and patient morbidity and mortality. Wrinkled surface topographies have previously demonstrated promising antifouling properties. Here we report a bioinspired strategy in which the actuation of silk fibroin produces tunable, wrinkled surface topographies on 2D shape memory polymer (SMP) substrates and investigate the influence of these topographies on biofilm formation. To mimic biofilm-associated infections related to the geometries of indwelling medical devices, silk wrinkles are produced on complex, 3D SMP architectures, and biofilm formation is evaluated. Using common biofilm-causing agents, smaller silk wrinkle wavelengths and amplitudes are found to significantly reduce biofilm formation, resulting in primarily isolated, single-cell bacteria on the 2D wrinkled surfaces. These single-cell bacteria are nearly completely eradicated by treatment with antibiotics, which are ineffective against control surfaces. Antibiotics are also physically incorporated into the 2D wrinkled surfaces, which resulted in a further significant reduction in bacterial adhesion. Lastly, silk wrinkled topographies are successfully applied on 3D architectures, and the wrinkled surfaces display a significant reduction in biofilm coverage compared to controls. The findings demonstrate the potential for biopolymer wrinkles on biomaterials to be used as antifouling surfaces for biofilm prevention.
AB - Bacterial biofilms on the surfaces of indwelling biomedical devices can cause long-term infection and patient morbidity and mortality. Wrinkled surface topographies have previously demonstrated promising antifouling properties. Here we report a bioinspired strategy in which the actuation of silk fibroin produces tunable, wrinkled surface topographies on 2D shape memory polymer (SMP) substrates and investigate the influence of these topographies on biofilm formation. To mimic biofilm-associated infections related to the geometries of indwelling medical devices, silk wrinkles are produced on complex, 3D SMP architectures, and biofilm formation is evaluated. Using common biofilm-causing agents, smaller silk wrinkle wavelengths and amplitudes are found to significantly reduce biofilm formation, resulting in primarily isolated, single-cell bacteria on the 2D wrinkled surfaces. These single-cell bacteria are nearly completely eradicated by treatment with antibiotics, which are ineffective against control surfaces. Antibiotics are also physically incorporated into the 2D wrinkled surfaces, which resulted in a further significant reduction in bacterial adhesion. Lastly, silk wrinkled topographies are successfully applied on 3D architectures, and the wrinkled surfaces display a significant reduction in biofilm coverage compared to controls. The findings demonstrate the potential for biopolymer wrinkles on biomaterials to be used as antifouling surfaces for biofilm prevention.
KW - antifouling surfaces
KW - biofilm inhibition
KW - shape memory polymer
KW - silk fibroin
KW - surface topographies
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U2 - 10.1002/admi.202400684
DO - 10.1002/admi.202400684
M3 - Article
AN - SCOPUS:85208417021
SN - 2196-7350
JO - Advanced Materials Interfaces
JF - Advanced Materials Interfaces
ER -