Avoiding common pitfalls in designing kinetic protocols for catalytic amyloid studies

Liam R. Marshall, Ivan V. Korendovych

Research output: Chapter in Book/Entry/PoemChapter

Abstract

Kinetic characterization of catalytic amyloids arguably presents a most challenging type of kinetic experiment where careful consideration of many factors is required. Here we outline common pitfalls in devising kinetic studies in such systems. Unlike the more specific protocols for various applications described in this volume, this chapter deals with general issues in setting up kinetic experiments that are incredibly important but often go without explicit mention in the specialized literature. The kinetic fundamentals described here can be also be of use to the enzymologists working with more traditional catalysts.

Original languageEnglish (US)
Title of host publicationPeptide Catalysts, Including Catalytic Amyloids
EditorsIvan V. Korendovych
PublisherAcademic Press Inc.
Pages1-13
Number of pages13
ISBN (Print)9780443236679
DOIs
StatePublished - Jan 2024

Publication series

NameMethods in Enzymology
Volume697
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Amyloids
  • Catalysis
  • Data quality
  • High-throughput screening
  • Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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