TY - JOUR
T1 - Automated micropipette aspiration of single cells
AU - Shojaei-Baghini, Ehsan
AU - Zheng, Yi
AU - Sun, Yu
N1 - Funding Information:
The authors thank John Nguyen for helpful discussions and thank Haijiao Liu and Prof. Craig Simmons for PAVIC cell preparation. The authors acknowledge financial support from the Natural Sciences and Engineering Research Council of Canada and the Canada Research Chairs Program.
PY - 2013/6
Y1 - 2013/6
N2 - This paper presents a system for mechanically characterizing single cells using automated micropipette aspiration. Using vision-based control and position control, the system controls a micromanipulator, a motorized translation stage, and a custom-built pressure system to position a micropipette (4 μm opening) to approach a cell, form a seal, and aspirate the cell into the micropipette for quantifying the cell's elastic and viscoelastic parameters as well as viscosity. Image processing algorithms were developed to provide controllers with real-time visual feedback and to accurately measure cell deformation behavior on line. Experiments on both solid-like and liquid-like cells demonstrated that the system is capable of efficiently performing single-cell micropipette aspiration and has low operator skill requirements.
AB - This paper presents a system for mechanically characterizing single cells using automated micropipette aspiration. Using vision-based control and position control, the system controls a micromanipulator, a motorized translation stage, and a custom-built pressure system to position a micropipette (4 μm opening) to approach a cell, form a seal, and aspirate the cell into the micropipette for quantifying the cell's elastic and viscoelastic parameters as well as viscosity. Image processing algorithms were developed to provide controllers with real-time visual feedback and to accurately measure cell deformation behavior on line. Experiments on both solid-like and liquid-like cells demonstrated that the system is capable of efficiently performing single-cell micropipette aspiration and has low operator skill requirements.
KW - Biological cell characterization
KW - Mechanical properties
KW - Micropipette aspiration
KW - Robotic cell manipulation
KW - Visual servoing
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U2 - 10.1007/s10439-013-0791-9
DO - 10.1007/s10439-013-0791-9
M3 - Article
C2 - 23508635
AN - SCOPUS:84886733367
SN - 0090-6964
VL - 41
SP - 1208
EP - 1216
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 6
ER -