Application of phase correlation to the montage synthesis and three-dimensional reconstruction of large tissue volumes from confocal laser scanning microscopy

Mohamed Adel Slamani, Andrzej Krol, Jacques Beaumont, Robert L. Price, Ioana L. Coman, Edward D. Lipson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We have implemented and tested a new automatic method for the montage synthesis and three-dimensional (3D) reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for CLSM. The sections were scanned horizontally (in-plane) and vertically (perpendicular to the optical planes) to produce "tiles" of a 3D volume. Phase correlation maximization was applied to the montage synthesis of in-plane tiles and 3D alignment of optical slices within a given physical section. The performance of the new method is evaluated.

Original languageEnglish (US)
Pages (from-to)106-112
Number of pages7
JournalMicroscopy and Microanalysis
Volume12
Issue number2
DOIs
StatePublished - Apr 2006

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Keywords

  • Confocal laser scanning microscopy
  • Image mosaicking
  • Montage synthesis
  • Phase correlation

ASJC Scopus subject areas

  • Instrumentation

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