Antimicrobial dendrimer active against Escherichia coli biofilms

Shuyu Hou; Chunhui Zhou; Zhigang Liu; Anne W. Young; Zhengshuang Shi; Dacheng Ren; Neville R. Kallenbach

doi:10.1016/j.bmcl.2009.07.077

Antimicrobial dendrimer active against Escherichia coli biofilms

Shuyu Hou, Chunhui Zhou, Zhigang Liu, Anne W. Young, Zhengshuang Shi, Dacheng Ren, Neville R. Kallenbach

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

We have investigated the ability of a previously reported antimicrobial peptide dendrimer (RW)_4D to inactivate Escherichia coli RP437 in planktonic culture and in biofilms. The results show that the dendrimer inhibits bacterial growth in both planktonic and biofilm states. Live/Dead staining assays reveal that most bacteria in a preformed biofilm lose viability after treatment with this peptide. This result is in marked contrast to most existing reports that antimicrobial peptides are ineffective against mature bacterial biofilms.

Original language	English (US)
Pages (from-to)	5478-5481
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	19
Issue number	18
DOIs	https://doi.org/10.1016/j.bmcl.2009.07.077
State	Published - Sep 15 2009

Keywords

Antimicrobial
Biofilm
Dendrimer peptide

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/j.bmcl.2009.07.077

Cite this

@article{5e8ef1bdcdaf40919bccb8fb369b4540,

title = "Antimicrobial dendrimer active against Escherichia coli biofilms",

abstract = "We have investigated the ability of a previously reported antimicrobial peptide dendrimer (RW)4D to inactivate Escherichia coli RP437 in planktonic culture and in biofilms. The results show that the dendrimer inhibits bacterial growth in both planktonic and biofilm states. Live/Dead staining assays reveal that most bacteria in a preformed biofilm lose viability after treatment with this peptide. This result is in marked contrast to most existing reports that antimicrobial peptides are ineffective against mature bacterial biofilms.",

keywords = "Antimicrobial, Biofilm, Dendrimer peptide",

author = "Shuyu Hou and Chunhui Zhou and Zhigang Liu and Young, {Anne W.} and Zhengshuang Shi and Dacheng Ren and Kallenbach, {Neville R.}",

note = "Funding Information: This work was supported by a grant from ONR (N00014-03-1-0129). We acknowledge NCRR/NIH for a Research Facilities Improvement Grant (C06 RR-16572) at NYU. Shuyu Hou was supported by a fellowship from the Syracuse Biomaterials Institute. We are also grateful to Dr. John S. Parkinson (University of Utah) for providing the strain of E. coli RP437.",

year = "2009",

month = sep,

day = "15",

doi = "10.1016/j.bmcl.2009.07.077",

language = "English (US)",

volume = "19",

pages = "5478--5481",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier",

number = "18",

}

TY - JOUR

T1 - Antimicrobial dendrimer active against Escherichia coli biofilms

AU - Hou, Shuyu

AU - Zhou, Chunhui

AU - Liu, Zhigang

AU - Young, Anne W.

AU - Shi, Zhengshuang

AU - Ren, Dacheng

AU - Kallenbach, Neville R.

N1 - Funding Information: This work was supported by a grant from ONR (N00014-03-1-0129). We acknowledge NCRR/NIH for a Research Facilities Improvement Grant (C06 RR-16572) at NYU. Shuyu Hou was supported by a fellowship from the Syracuse Biomaterials Institute. We are also grateful to Dr. John S. Parkinson (University of Utah) for providing the strain of E. coli RP437.

PY - 2009/9/15

Y1 - 2009/9/15

N2 - We have investigated the ability of a previously reported antimicrobial peptide dendrimer (RW)4D to inactivate Escherichia coli RP437 in planktonic culture and in biofilms. The results show that the dendrimer inhibits bacterial growth in both planktonic and biofilm states. Live/Dead staining assays reveal that most bacteria in a preformed biofilm lose viability after treatment with this peptide. This result is in marked contrast to most existing reports that antimicrobial peptides are ineffective against mature bacterial biofilms.

AB - We have investigated the ability of a previously reported antimicrobial peptide dendrimer (RW)4D to inactivate Escherichia coli RP437 in planktonic culture and in biofilms. The results show that the dendrimer inhibits bacterial growth in both planktonic and biofilm states. Live/Dead staining assays reveal that most bacteria in a preformed biofilm lose viability after treatment with this peptide. This result is in marked contrast to most existing reports that antimicrobial peptides are ineffective against mature bacterial biofilms.

KW - Antimicrobial

KW - Biofilm

KW - Dendrimer peptide

UR - http://www.scopus.com/inward/record.url?scp=68949122716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68949122716&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2009.07.077

DO - 10.1016/j.bmcl.2009.07.077

M3 - Article

C2 - 19682902

AN - SCOPUS:68949122716

SN - 0960-894X

VL - 19

SP - 5478

EP - 5481

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 18

ER -

Antimicrobial dendrimer active against Escherichia coli biofilms

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this