Antibacterial and Cytocompatible pH-Responsive Peptide Hydrogel

Dona Imanga Upamadi Edirisinghe; Areetha D’Souza; Maryam Ramezani; Robert J. Carroll; Quenten Chicón; Cheyene L. Muenzel; Jonathan Soule; Mary Beth Browning Monroe; Alison E. Patteson; Olga V. Makhlynets

doi:10.3390/molecules28114390

Antibacterial and Cytocompatible pH-Responsive Peptide Hydrogel

Dona Imanga Upamadi Edirisinghe, Areetha D’Souza, Maryam Ramezani, Robert J. Carroll, Quenten Chicón, Cheyene L. Muenzel, Jonathan Soule, Mary Beth Browning Monroe, Alison E. Patteson, Olga V. Makhlynets

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A short peptide, FHHF-11, was designed to change stiffness as a function of pH due to changing degree of protonation of histidines. As pH changes in the physiologically relevant range, G′ was measured at 0 Pa (pH 6) and 50,000 Pa (pH 8). This peptide-based hydrogel is antimicrobial and cytocompatible with skin cells (fibroblasts). It was demonstrated that the incorporation of unnatural AzAla tryptophan analog residue improves the antimicrobial properties of the hydrogel. The material developed can have a practical application and be a paradigm shift in the approach to wound treatment, and it will improve healing outcomes for millions of patients each year.

Original language	English (US)
Article number	4390
Journal	Molecules
Volume	28
Issue number	11
DOIs	https://doi.org/10.3390/molecules28114390
State	Published - Jun 2023

Keywords

antimicrobial
cytocompatible
hydrogel
pH sensitive
rheology
self-healing
wound healing

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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10.3390/molecules28114390

Cite this

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title = "Antibacterial and Cytocompatible pH-Responsive Peptide Hydrogel",

abstract = "A short peptide, FHHF-11, was designed to change stiffness as a function of pH due to changing degree of protonation of histidines. As pH changes in the physiologically relevant range, G′ was measured at 0 Pa (pH 6) and 50,000 Pa (pH 8). This peptide-based hydrogel is antimicrobial and cytocompatible with skin cells (fibroblasts). It was demonstrated that the incorporation of unnatural AzAla tryptophan analog residue improves the antimicrobial properties of the hydrogel. The material developed can have a practical application and be a paradigm shift in the approach to wound treatment, and it will improve healing outcomes for millions of patients each year.",

keywords = "antimicrobial, cytocompatible, hydrogel, pH sensitive, rheology, self-healing, wound healing",

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doi = "10.3390/molecules28114390",

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AU - Edirisinghe, Dona Imanga Upamadi

AU - D’Souza, Areetha

AU - Ramezani, Maryam

AU - Carroll, Robert J.

AU - Chicón, Quenten

AU - Muenzel, Cheyene L.

AU - Soule, Jonathan

AU - Monroe, Mary Beth Browning

AU - Patteson, Alison E.

AU - Makhlynets, Olga V.

PY - 2023/6

Y1 - 2023/6

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AB - A short peptide, FHHF-11, was designed to change stiffness as a function of pH due to changing degree of protonation of histidines. As pH changes in the physiologically relevant range, G′ was measured at 0 Pa (pH 6) and 50,000 Pa (pH 8). This peptide-based hydrogel is antimicrobial and cytocompatible with skin cells (fibroblasts). It was demonstrated that the incorporation of unnatural AzAla tryptophan analog residue improves the antimicrobial properties of the hydrogel. The material developed can have a practical application and be a paradigm shift in the approach to wound treatment, and it will improve healing outcomes for millions of patients each year.

KW - antimicrobial

KW - cytocompatible

KW - hydrogel

KW - pH sensitive

KW - rheology

KW - self-healing

KW - wound healing

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Antibacterial and Cytocompatible pH-Responsive Peptide Hydrogel

Abstract

Keywords

ASJC Scopus subject areas

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