Analyzing near infrared scattering from human skin to monitor changes in hematocrit

Joseph Chaiken, Bin Deng, Jerry Goodisman, George Shaheen, R. J. Bussjager

Research output: Chapter in Book/Entry/PoemConference contribution

4 Scopus citations


The leading preventable cause of death, world-wide, civilian or military, for all people between the ages of 18-45 is undetected internal hemorrhage. Autonomic compensation mechanisms mask changes such as e.g. hematocrit fluctuations that could give early warning if only they could be monitored continuously with reasonable degrees of precision and relative accuracy. Probing tissue with near infrared radiation (NIR) simultaneously produces remitted fluorescence and Raman scattering (IE) plus Rayleigh/Mie light scattering (EE) that noninvasively give chemical and physical information about the materials and objects within. We model tissue as a three-phase system: plasma and red blood cell (RBC) phases that are mobile and a static tissue phase. In vivo, any volume of tissue naturally experiences spatial and temporal fluctuations of blood plasma and RBC content. Plasma and RBC fractions may be discriminated from each other on the basis of their physical, chemical and optical properties. Thus IE and EE from NIR probing yield information about these fractions. Assuming there is no void volume in viable tissue, or that void volume is constant, changes in plasma and RBC volume fractions may be calculated from simultaneous measurements of the two observables, EE and IE. In a previously published analysis we showed the underlying phenomenology but did not provide an algorithm for calculating volume fractions from experimental data. Here we present a simple analysis that allows continuous monitoring of fluid fraction and hematocrit (Hct) changes by measuring IE and EE, and apply it to some experimental in vivo measurements.

Original languageEnglish (US)
Title of host publicationBiomedical Vibrational Spectroscopy V
Subtitle of host publicationAdvances in Research and Industry
StatePublished - 2012
EventBiomedical Vibrational Spectroscopy V: Advances in Research and Industry - San Francisco, CA, United States
Duration: Jan 21 2012Jan 22 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherBiomedical Vibrational Spectroscopy V: Advances in Research and Industry
Country/TerritoryUnited States
CitySan Francisco, CA


  • Mie
  • Noninvasive
  • Raman
  • Rayleigh
  • blood
  • fluorescence
  • hematocrit
  • hemorrhage

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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