Analyzing near-infrared scattering from human skin to monitor changes in hematocrit

Joseph Chaiken, Bin Deng, Jerry Goodisman, George Shaheen, Rebecca J. Bussjager

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Probing tissue with near-infrared radiation (NIR) simultaneously produces remitted fluorescence and Raman scattering (IE) plus RayleighMie light scattering (EE) that noninvasively give chemical and physical information about the materials and objects within. We model tissue as a three-phase system: plasma and red blood cell (RBC) phases that are mobile and a static tissue phase. In vivo, any volume of tissue naturally experiences spatial and temporal fluctuations of blood plasma and RBC content. Plasma and RBC fractions may be discriminated from each other on the basis of their physical, chemical, and optical properties. Thus, IE and EE from NIR probing yield information about these fractions. Assuming there is no void volume in viable tissue, or that void volume is constant, changes in plasma and RBC volume fractions may be calculated from simultaneous measurements of the two observables, EE and IE. In a previously published analysis we showed the underlying phenomenology but did not provide an algorithm for calculating volume fractions from experimental data. Now, we present a simple analysis that allows monitoring of fluid fraction and hematocrit (Hct) changes by measuring IE and EE, and apply it to some experimental in vivo measurements.

Original languageEnglish (US)
Article number097005
JournalJournal of Biomedical Optics
Volume16
Issue number9
DOIs
StatePublished - Sep 2011

Fingerprint

hematocrit
erythrocytes
Skin
Blood
Scattering
Tissue
Infrared radiation
near infrared radiation
Plasmas
scattering
voids
Volume fraction
blood plasma
phenomenology
chemical properties
Light scattering
Chemical properties
Raman scattering
light scattering
Optical properties

Keywords

  • continuous hematocrit
  • internal hemorrhage
  • noninvasive

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Analyzing near-infrared scattering from human skin to monitor changes in hematocrit. / Chaiken, Joseph; Deng, Bin; Goodisman, Jerry; Shaheen, George; Bussjager, Rebecca J.

In: Journal of Biomedical Optics, Vol. 16, No. 9, 097005, 09.2011.

Research output: Contribution to journalArticle

Chaiken, Joseph ; Deng, Bin ; Goodisman, Jerry ; Shaheen, George ; Bussjager, Rebecca J. / Analyzing near-infrared scattering from human skin to monitor changes in hematocrit. In: Journal of Biomedical Optics. 2011 ; Vol. 16, No. 9.
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