Analysis of the anisotropy decay of trans-parinaric acid in lipid bilayers

A. Ruggiero, B. Hudson

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

An analysis is presented of the complex anisotropy behavior of trans-parinaric acid in single component DEPC lipid bilayers. It is shown that a model involving two species with distinct lifetime and motional behavior is required, and is adequate, to explain the observed data. In particular, the observed increase in the anisotropy at long times demonstrates the presence of a species with a long fluorescence lifetime that has a high anisotropy. The time dependence of the anisotropy for these two environments is treated using both a purely mathematical sum of exponentials and a constrained fit based on an approximate solution of the anisotropic diffusion problem. In this latter model the anisotropy is described in terms of the second and fourth rank order parameters, (P2) and (P4), and a single dynamical parameter, D1, the perpendicular diffusion coefficient for this uniaxial probe. The parameters of both models are accurately determined from the fits to the data when two environments coexist and an association is made between lifetime components and distinct rotational sites. The values of the parameters obtained demonstrate the "solid-like" and "fluidlike" nature of these two coexisting environments.

Original languageEnglish (US)
Pages (from-to)1125-1135
Number of pages11
JournalBiophysical Journal
Volume55
Issue number6
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'Analysis of the anisotropy decay of trans-parinaric acid in lipid bilayers'. Together they form a unique fingerprint.

  • Cite this