Analysis of microsomal flavoproteins from Phycomyces sporangiophores: Candidates for the blue-light photoreceptor

J. A. Pollock, E. D. Lipson, D. T. Sullivan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To help identify components of the blue-light photoreceptor system for phototropism in Phycomyces blakesleeanus Bgff., proteins from a microsomal fraction obtained from synchronous sporangiophores were studied. By two-dimensional gel electrophoresis, two proteins (FP1, FP2) with covalently bound flavins were found. FP1 has a molecular weight of 71 000 and an isoelectric point of 6.6; FP2 has a molecular weight of 59 000 and an isoelectric point of 6.5. These flavoproteins were purified by column chromatography and gel filtration while assaying for flavins by fluorescence. The relative concentrations of FP1 and FP2 were affected by light applied during growth. These flavoproteins are likely components of the blue-light photoreceptor complex mediating phototropism in Phycomyces.

Original languageEnglish (US)
Pages (from-to)506-516
Number of pages11
JournalPlanta
Volume163
Issue number4
DOIs
StatePublished - Apr 1985

Fingerprint

Phycomyces
flavoproteins
phototropism
Flavoproteins
flavins
Phototropism
isoelectric point
blue light
photoreceptors
Flavins
Phycomyces blakesleeanus
Isoelectric Point
molecular weight
Light
two-dimensional gel electrophoresis
Molecular Weight
chromatography
proteins
Electrophoresis, Gel, Two-Dimensional
gels

Keywords

  • Blue-light photoreceptor
  • Flavoprotein
  • Phototropism
  • Phycomyces

ASJC Scopus subject areas

  • Plant Science

Cite this

Analysis of microsomal flavoproteins from Phycomyces sporangiophores : Candidates for the blue-light photoreceptor. / Pollock, J. A.; Lipson, E. D.; Sullivan, D. T.

In: Planta, Vol. 163, No. 4, 04.1985, p. 506-516.

Research output: Contribution to journalArticle

Pollock, J. A. ; Lipson, E. D. ; Sullivan, D. T. / Analysis of microsomal flavoproteins from Phycomyces sporangiophores : Candidates for the blue-light photoreceptor. In: Planta. 1985 ; Vol. 163, No. 4. pp. 506-516.
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