TY - JOUR
T1 - Analysis of genetic variation in selected stocks of hatchery flounder, Paralichthys olivaceus, using AFLP markers
AU - Liu, Yun Guo
AU - Chen, Song Lin
AU - Li, Ba Fang
AU - Wang, Zhi Jian
AU - Liu, Zhanjiang
N1 - Funding Information:
We would like to express our gratitude to Dr. Zhiqin Yue and Weiji Wang, from Yellow Sea Fisheries Research Institute, CAFS, Dr. Xiande Liu, from Institute of Oceanography, CAS, and Dr. Chunhua Mu, from Ocean University of China for their kind assistance on this study. This project was supported by grants from National Nature Science Foundation of China (30413403) and State 863 High-Technology R&D Project of China (2002AA626010).
PY - 2005/10
Y1 - 2005/10
N2 - Amplified fragment length polymorphism (AFLP) analysis was performed in order to evaluate genetic characteristics of one common population and two selective hatchery populations of flounder Paralichthys olivaceus. A group of 60 genotypes belonging to three populations was screened using 10 different AFLP primer combinations. A total of 491 loci were produced in the three studied populations. The loci of 65.78%, 61.47% and 60.92% were polymorphic over all the genotypes tested in common, susceptible and resistant populations, respectively. The number of polymorphic loci detected by single primer combination ranged from 21 to 43. The average heterozygosity of common, susceptible and resistant populations was 0.1656, 0.1609 and 0.1586, respectively, which showed no significant difference. Compared with the common population, the two selective hatchery populations, susceptible and resistant, showed significant genetic differences including a smaller (P < 0.05) number of total loci, a smaller (P < 0.05) number of total polymorphic loci and a smaller (P < 0.05) percentage of low frequency (0-0.2) polymorphic loci. AFLP banding pattern was transformed into binary data and matrices were processed with POPGENE and TFPGA software. Similarity relationships were described graphically by a dendrogram, which clustered the three populations. The AFLP fingerprinting technique was confirmed to be a reproducible and sensitive tool for the study of population genetics of flounder. The present study confirmed that it was important to detect the genetic variability of the selective hatchery populations for the conservation of natural flounder resources.
AB - Amplified fragment length polymorphism (AFLP) analysis was performed in order to evaluate genetic characteristics of one common population and two selective hatchery populations of flounder Paralichthys olivaceus. A group of 60 genotypes belonging to three populations was screened using 10 different AFLP primer combinations. A total of 491 loci were produced in the three studied populations. The loci of 65.78%, 61.47% and 60.92% were polymorphic over all the genotypes tested in common, susceptible and resistant populations, respectively. The number of polymorphic loci detected by single primer combination ranged from 21 to 43. The average heterozygosity of common, susceptible and resistant populations was 0.1656, 0.1609 and 0.1586, respectively, which showed no significant difference. Compared with the common population, the two selective hatchery populations, susceptible and resistant, showed significant genetic differences including a smaller (P < 0.05) number of total loci, a smaller (P < 0.05) number of total polymorphic loci and a smaller (P < 0.05) percentage of low frequency (0-0.2) polymorphic loci. AFLP banding pattern was transformed into binary data and matrices were processed with POPGENE and TFPGA software. Similarity relationships were described graphically by a dendrogram, which clustered the three populations. The AFLP fingerprinting technique was confirmed to be a reproducible and sensitive tool for the study of population genetics of flounder. The present study confirmed that it was important to detect the genetic variability of the selective hatchery populations for the conservation of natural flounder resources.
KW - AFLP marker
KW - Flounder
KW - Genetic characteristics
KW - Genetic variation
KW - Paralichthys olivaceus
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U2 - 10.1016/j.bse.2005.02.008
DO - 10.1016/j.bse.2005.02.008
M3 - Article
AN - SCOPUS:21644490421
SN - 0305-1978
VL - 33
SP - 993
EP - 1005
JO - Biochemical Systematics and Ecology
JF - Biochemical Systematics and Ecology
IS - 10
ER -