Analysis of apolipoprotein genes and their involvement in disease response of channel catfish after bacterial infection

Yujia Yang, Qiang Fu, Tao Zhou, Yun Li, Shikai Liu, Qifan Zeng, Xiaozhu Wang, Yulin Jin, Changxu Tian, Zhenkui Qin, Rex Dunham, Zhanjian "John" Liu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Apolipoproteins are protein component of plasma lipoproteins. They exert crucial roles in lipoprotein metabolism and serve as enzyme cofactors, receptor ligands, and lipid transfer carriers in mammals. In teleosts, apolipoproteins are also involved in diverse processes including embryonic and ontogenic development, liver and digestive system organogenesis, and innate immunity. In this study, we identified a set of 19 apolipoprotein genes in channel catfish (Ictalurus punctatus). Phylogenetic analysis and syntenic analysis were conducted to determine their identities and evolutionary relationships. The expression signatures of apolipoproteins in channel catfish were determined in healthy tissues and after infections with two major bacterial pathogens, Edwardsiella ictaluri and Flavobacterium columnare. In healthy channel catfish, most apolipoprotein genes exhibited tissue-specific expression patterns in channel catfish. After ESC and columnaris infections, 5 and 7 apolipoprotein genes were differentially expressed respectively, which presented a pathogen-specific and time-dependent pattern of regulation. After ESC infection, three exchangeable apolipoproteins (apoA-IB, apoC-I, and apoE-B) were suppressed in catfish intestine, while two nonexchangeable apolipoproteins (apoB-A and apoB-B) were slightly up-regulated. After columnaris infection, apoB-B, apoD-B, and apoE-A were significantly down-regulated in catfish gill, while apoF, apoL-IV, apoO-like, and apo-14 kDa showed significantly up-regulation. Taken together, these results suggested that apolipoprotein genes may play significant roles in innate immune responses to bacterial pathogens in channel catfish.

Original languageEnglish (US)
Pages (from-to)464-470
Number of pages7
JournalDevelopmental and Comparative Immunology
Volume67
DOIs
StatePublished - Feb 1 2017
Externally publishedYes

Fingerprint

Ictaluridae
Apolipoproteins
Bacterial Infections
Genes
Apolipoproteins B
Catfishes
Apolipoproteins E
Infection
Innate Immunity
Lipoproteins
Edwardsiella ictaluri
Apolipoproteins C
Apolipoproteins A
Flavobacterium
Digestive System
Organogenesis
Coenzymes
Intestines
Embryonic Development
Blood Proteins

Keywords

  • Disease
  • Fish
  • Genome
  • Immune response
  • Innate immunity

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

Analysis of apolipoprotein genes and their involvement in disease response of channel catfish after bacterial infection. / Yang, Yujia; Fu, Qiang; Zhou, Tao; Li, Yun; Liu, Shikai; Zeng, Qifan; Wang, Xiaozhu; Jin, Yulin; Tian, Changxu; Qin, Zhenkui; Dunham, Rex; Liu, Zhanjian "John".

In: Developmental and Comparative Immunology, Vol. 67, 01.02.2017, p. 464-470.

Research output: Contribution to journalArticle

Yang, Yujia ; Fu, Qiang ; Zhou, Tao ; Li, Yun ; Liu, Shikai ; Zeng, Qifan ; Wang, Xiaozhu ; Jin, Yulin ; Tian, Changxu ; Qin, Zhenkui ; Dunham, Rex ; Liu, Zhanjian "John". / Analysis of apolipoprotein genes and their involvement in disease response of channel catfish after bacterial infection. In: Developmental and Comparative Immunology. 2017 ; Vol. 67. pp. 464-470.
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AU - Yang, Yujia

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AU - Zhou, Tao

AU - Li, Yun

AU - Liu, Shikai

AU - Zeng, Qifan

AU - Wang, Xiaozhu

AU - Jin, Yulin

AU - Tian, Changxu

AU - Qin, Zhenkui

AU - Dunham, Rex

AU - Liu, Zhanjian "John"

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AB - Apolipoproteins are protein component of plasma lipoproteins. They exert crucial roles in lipoprotein metabolism and serve as enzyme cofactors, receptor ligands, and lipid transfer carriers in mammals. In teleosts, apolipoproteins are also involved in diverse processes including embryonic and ontogenic development, liver and digestive system organogenesis, and innate immunity. In this study, we identified a set of 19 apolipoprotein genes in channel catfish (Ictalurus punctatus). Phylogenetic analysis and syntenic analysis were conducted to determine their identities and evolutionary relationships. The expression signatures of apolipoproteins in channel catfish were determined in healthy tissues and after infections with two major bacterial pathogens, Edwardsiella ictaluri and Flavobacterium columnare. In healthy channel catfish, most apolipoprotein genes exhibited tissue-specific expression patterns in channel catfish. After ESC and columnaris infections, 5 and 7 apolipoprotein genes were differentially expressed respectively, which presented a pathogen-specific and time-dependent pattern of regulation. After ESC infection, three exchangeable apolipoproteins (apoA-IB, apoC-I, and apoE-B) were suppressed in catfish intestine, while two nonexchangeable apolipoproteins (apoB-A and apoB-B) were slightly up-regulated. After columnaris infection, apoB-B, apoD-B, and apoE-A were significantly down-regulated in catfish gill, while apoF, apoL-IV, apoO-like, and apo-14 kDa showed significantly up-regulation. Taken together, these results suggested that apolipoprotein genes may play significant roles in innate immune responses to bacterial pathogens in channel catfish.

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KW - Fish

KW - Genome

KW - Immune response

KW - Innate immunity

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