An in vitro vascular chip using 3D printing-enabled hydrogel casting

Liang Yang, Shivkumar Vishnempet Shridhar, Melissa Gerwitz, Pranav Soman

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

An important unsolved challenge in tissue engineering has been the inability to replicate the geometry and function of vascular networks and blood vessels. Here, we engineer a user-defined 3D microfluidic vascular channel using 3D printing-enabled hydrogel casting. First, a hollow L-shaped channel is developed using a template casting process. In this process, murine 10T1/2 cells are encapsulated within gelatin methacrylate (GelMA) hydrogel usingUVphotocrosslinking, and upon removal of the template results in a hollow channel within GelMA. Second, human umbilical vein endothelial cells (HUVECs) were cultured within the channel and immunostaining was used to visualize endothelial monolayers. Third, diffusion/permeability studies on endothelialized channels were carried out to demonstrate the barrier function ofHUVECmonolayer. Taken together, we develop a facile, cytocompatible and rapid approach to engineer a user-defined multicellular vascular chip that could be potentially useful in developing new vascular model systems.

Original languageEnglish (US)
Article number035015
JournalBiofabrication
Volume8
Issue number3
DOIs
StatePublished - Aug 26 2016

Keywords

  • 3D printing
  • encapsulation
  • gelatin methacrylate
  • hydrogel
  • microfluidic

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

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