An in vitro vascular chip using 3D printing-enabled hydrogel casting

Liang Yang, Shivkumar Vishnempet Shridhar, Melissa Gerwitz, Pranav Soman

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


An important unsolved challenge in tissue engineering has been the inability to replicate the geometry and function of vascular networks and blood vessels. Here, we engineer a user-defined 3D microfluidic vascular channel using 3D printing-enabled hydrogel casting. First, a hollow L-shaped channel is developed using a template casting process. In this process, murine 10T1/2 cells are encapsulated within gelatin methacrylate (GelMA) hydrogel usingUVphotocrosslinking, and upon removal of the template results in a hollow channel within GelMA. Second, human umbilical vein endothelial cells (HUVECs) were cultured within the channel and immunostaining was used to visualize endothelial monolayers. Third, diffusion/permeability studies on endothelialized channels were carried out to demonstrate the barrier function ofHUVECmonolayer. Taken together, we develop a facile, cytocompatible and rapid approach to engineer a user-defined multicellular vascular chip that could be potentially useful in developing new vascular model systems.

Original languageEnglish (US)
Article number035015
Issue number3
StatePublished - Aug 26 2016


  • 3D printing
  • encapsulation
  • gelatin methacrylate
  • hydrogel
  • microfluidic

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering


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