Alternative complement pathway of channel catfish (Ictalurus punctatus): Molecular characterization, mapping and expression analysis of factors Bf/C2 and Df

Zunchun Zhou, Hong Liu, Shikai Liu, Fanyue Sun, Eric Peatman, Huseyin Kucuktas, Ludmilla Kaltenboeck, Tingting Feng, Hao Zhang, Donghong Niu, Jianguo Lu, Geoff Waldbieser, Zhanjiang Liu

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The complement system is important in both innate and adaptive host defense against microbial infection in vertebrates. It contains three pathways: the classical, alternative, and lectin pathways. Complement component factors B and D are two crucial proteases in the alternative pathway. In this study, the genes of complement factors Bf/C2 and Df from channel catfish, Ictalurus punctatus were identified and characterized. Two complement factor B-related genes, Bf/C2A and Bf/C2B, and factor D gene Df were identified. Phylogenetic analysis suggested that Bf/C2A and Bf/C2B is likely orthologous to factor B and factor C2, respectively. Southern blot results suggested that these three genes are all single-copy genes in the catfish genome. The catfish Bf/C2A, Bf/C2B and Df genes were genetically mapped on linkage group 3, 20 and 29, respectively. Bf/C2A and Bf/C2B are highly expressed in liver and kidney, while Df is highly expressed in gill and spleen. After infection with Edwardsiella ictaluri, the expression of Bf/C2A, Bf/C2B and Df genes were found to be remarkably induced in the gill, liver, spleen and kidney at some sampling times, indicating that these three complement factors play a pivotal role in immune responses after the bacterial infection in catfish.

Original languageEnglish (US)
Pages (from-to)186-195
Number of pages10
JournalFish and Shellfish Immunology
Volume32
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • Catfish
  • Complement
  • Factor B
  • Factor C2
  • Innate immunity

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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    Zhou, Z., Liu, H., Liu, S., Sun, F., Peatman, E., Kucuktas, H., Kaltenboeck, L., Feng, T., Zhang, H., Niu, D., Lu, J., Waldbieser, G., & Liu, Z. (2012). Alternative complement pathway of channel catfish (Ictalurus punctatus): Molecular characterization, mapping and expression analysis of factors Bf/C2 and Df. Fish and Shellfish Immunology, 32(1), 186-195. https://doi.org/10.1016/j.fsi.2011.11.012