All-trans-retinal is the chromophore bound to the photoreceptor of the alga Chlamydomonas reinhardtii.

F. Derguini, P. Mazur, K. Nakanishi, D. M. Starace, J. Saranak, K. W. Foster

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Rhodopsin is the general name for a family of visual pigments that receive light and transmit this signal to the rest of an organism. Chlamydomonas reinhardtii is a unicellular eukaryote whose light-tracking system consists of a single eye. Through spectral studies of Chlamydomonas' reaction to light of different wavelengths (action spectroscopy), it has been shown in vivo that the photoreceptor of Chlamydomonas is functionally similar to vertebrate rhodopsin. We seek to characterize the photoreceptor further by identifying the molecule that is incorporated into the rhodopsin of Chlamydomonas forming the chromophore. High performance liquid chromatography analysis of organic extracts of retinaloximes from membrane fractions enriched in eye-spots and in cells virtually free of interfering carotenoids identified syn-all-trans as the existing retinaloxime isomer. We conclude that all-trans-retinal is the native molecule that is available to be incorporated into the rhodopsin of Chlamydomonas and therefore forms the functioning chromophore on binding.

Original languageEnglish (US)
Pages (from-to)1017-1021
Number of pages5
JournalPhotochemistry and Photobiology
Volume54
Issue number6
StatePublished - Dec 1991

Fingerprint

Chlamydomonas
Chlamydomonas reinhardtii
photoreceptors
Rhodopsin
algae
Chromophores
Algae
chromophores
visual pigments
Light
eukaryotes
carotenoids
vertebrates
liquid chromatography
organisms
Molecules
Retinal Pigments
molecules
High performance liquid chromatography
Carotenoids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Derguini, F., Mazur, P., Nakanishi, K., Starace, D. M., Saranak, J., & Foster, K. W. (1991). All-trans-retinal is the chromophore bound to the photoreceptor of the alga Chlamydomonas reinhardtii. Photochemistry and Photobiology, 54(6), 1017-1021.

All-trans-retinal is the chromophore bound to the photoreceptor of the alga Chlamydomonas reinhardtii. / Derguini, F.; Mazur, P.; Nakanishi, K.; Starace, D. M.; Saranak, J.; Foster, K. W.

In: Photochemistry and Photobiology, Vol. 54, No. 6, 12.1991, p. 1017-1021.

Research output: Contribution to journalArticle

Derguini, F, Mazur, P, Nakanishi, K, Starace, DM, Saranak, J & Foster, KW 1991, 'All-trans-retinal is the chromophore bound to the photoreceptor of the alga Chlamydomonas reinhardtii.', Photochemistry and Photobiology, vol. 54, no. 6, pp. 1017-1021.
Derguini F, Mazur P, Nakanishi K, Starace DM, Saranak J, Foster KW. All-trans-retinal is the chromophore bound to the photoreceptor of the alga Chlamydomonas reinhardtii. Photochemistry and Photobiology. 1991 Dec;54(6):1017-1021.
Derguini, F. ; Mazur, P. ; Nakanishi, K. ; Starace, D. M. ; Saranak, J. ; Foster, K. W. / All-trans-retinal is the chromophore bound to the photoreceptor of the alga Chlamydomonas reinhardtii. In: Photochemistry and Photobiology. 1991 ; Vol. 54, No. 6. pp. 1017-1021.
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