AGPDMS electrodes for single cell impedance spectroscopy

Yuan Wei, Mark A. Cachia, John Nguyen, Zhensong Xu, Yi Zheng, Chen Wang, Yu Sun

Research output: Chapter in Book/Entry/PoemConference contribution

Abstract

This paper presents a microfluidic device with wide channels and embedded AgPDMS electrodes for measuring the electrical properties of single cells. The work demonstrates the feasibility of using a large channel design and embedded electrodes for impedance spectroscopy to circumvent issues such as channel clogging and limited device re-usability. AgPDMS electrodes were formed on channel sidewalls for impedance detection and cell characterization. Equivalent circuit models were used to interpret multi-frequency impedance data to quantify each cell's cytoplasm conductivity and specific membrane capacitance. Comparisons were made by measuring two leukemia cell lines (AML-2 and HL-60) which were found to have different cytoplasm conductivity values (0.29 ± 0.15 S/m vs. 0.47 ± 0.20 S/m) and specific membrane capacitance values (41 ± 25 mF/m2 vs. 55 ± 26 mF/m2).

Original languageEnglish (US)
Title of host publicationMEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages701-704
Number of pages4
ISBN (Electronic)9781509019731
DOIs
StatePublished - Feb 26 2016
Externally publishedYes
Event29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China
Duration: Jan 24 2016Jan 28 2016

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2016-February
ISSN (Print)1084-6999

Conference

Conference29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
Country/TerritoryChina
CityShanghai
Period1/24/161/28/16

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint

Dive into the research topics of 'AGPDMS electrodes for single cell impedance spectroscopy'. Together they form a unique fingerprint.

Cite this