Aging-related changes in the iron status of skeletal muscle

Keith C Deruisseau, Young Min Park, Lara R. DeRuisseau, Patrick M. Cowley, Christopher H. Fazen, Robert Patrick Doyle

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The rise in non-heme iron (NHI) concentration observed in skeletal muscle of aging rodents is thought to contribute to the development of sarcopenia. The source of the NHI has not been identified, nor have the physiological ramifications of elevated iron status in aged muscle been directly examined. Therefore, we assessed plantaris NHI and heme iron (HI) levels in addition to expression of proteins involved in iron uptake (transferrin receptor-1; TfR1), storage (ferritin), export (ferroportin; FPN), and regulation (iron regulatory protein-1 (IRP1) and -2 (IRP2)) of male F344xBN F1 rats (n = 10/group) of various ages (8, 18, 28, 32, and 36. months) to further understand iron regulation in aging muscle. In a separate experiment, iron chelator (pyridoxal isonicotinoyl hydrazone; PIH) or vehicle was administered to male F344xBN F1 rats (n = 8/group) beginning at 30. months of age to assess the impact on plantaris muscle mass and function at ~. 36. months of age. Principle findings revealed the increased NHI concentration in old age was consistent with concentrating effects of muscle atrophy and reduction in HI levels, with no change in the total iron content of the muscle. The greatest increase in muscle iron content occurred during the period of animal growth and was associated with downregulation of TfR1 and IRP2 expression. Ferritin upregulation did not occur until senescence and the protein remained undetectable during the period of muscle iron content elevation. Lastly, administration of PIH did not significantly (p > 0.05) impact NHI or measures of muscle atrophy or contractile function. In summary, this study confirms that the elevated NHI concentration in old age is largely due to the loss in muscle mass. The increased muscle iron content during aging does not appear to associate with cytosolic ferritin storage, but the functional consequences of elevated iron status in old age remains to be determined.

Original languageEnglish (US)
Pages (from-to)1294-1302
Number of pages9
JournalExperimental Gerontology
Volume48
Issue number11
DOIs
StatePublished - Nov 2013

Fingerprint

Muscle
Skeletal Muscle
Iron
Aging of materials
Muscles
Ferritins
Muscular Atrophy
Heme
Iron Regulatory Protein 2
Rats
Iron Regulatory Protein 1
Sarcopenia
Transferrin Receptors
Chelating Agents
Rodentia
Animals
Proteins
Up-Regulation
Down-Regulation
Age Groups

Keywords

  • Muscle aging
  • Muscle function
  • Pyridoxal isonicotinoyl hydrazone
  • Sarcopenia
  • Skeletal muscle iron regulation

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Genetics
  • Molecular Biology

Cite this

Aging-related changes in the iron status of skeletal muscle. / Deruisseau, Keith C; Park, Young Min; DeRuisseau, Lara R.; Cowley, Patrick M.; Fazen, Christopher H.; Doyle, Robert Patrick.

In: Experimental Gerontology, Vol. 48, No. 11, 11.2013, p. 1294-1302.

Research output: Contribution to journalArticle

Deruisseau, Keith C ; Park, Young Min ; DeRuisseau, Lara R. ; Cowley, Patrick M. ; Fazen, Christopher H. ; Doyle, Robert Patrick. / Aging-related changes in the iron status of skeletal muscle. In: Experimental Gerontology. 2013 ; Vol. 48, No. 11. pp. 1294-1302.
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