Affinities of the nucleocapsid protein for variants of SL3 RNA in HIV-1

Andrew C. Paoletti, Michael F. Shubsda, Bruce S. Hudson, Philip N. Borer

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Efficient packaging of genomic RNA into new HIV-1 virus particles requires that nucleocapsid domains of precursor proteins bind the SL3 tetraloop (G317-G-A-G320) from the 5′-untranslated region. This paper presents the affinities of 35 RNA variants of SL3 for the mature 55mer NC protein, as measured by fluorescence quenching of tryptophan-37 in the protein by nucleobases. The 1:1 complexes that form in 0.2 M NaCl have dissociation constants ranging from 8 nM (GGUG) to 20 μM (GAUA). The highly conserved (GGAG) sequence for the wild type is not the most stable (Kd = 28 nM), suggesting that other selective pressures beyond the stability of the complex must be satisfied. The leading requirement for strong interaction is for G320, followed closely by G318. Replacing either with U, A, or C reduces affinity by a factor of 15-120. NC-domains from multiple proteins combine to recognize unpaired G2-loci, where two guanines are in close proximity. We have previously measured affinities of the NC protein for the important stem-loops of the major packaging domain [Shubsda, M. F., Paoletti, A. C., Hudson, B. S., and Borer, P. N. (2002) Biochemistry 41, 5276-82]. Comparison with the present work shows that the nature of the stem also modulates NC-RNA interactions. Placing the G2-loci from the apical SL2 or SL1 loops on the SL3 stem increases affinity by a factor of 2-3, while placing the SL4 loop on the SL3 stem reduces affinity 50-fold. These results are interesting in the context of RNA-protein interaction, as well as for the discovery of antiNC agents for AIDS therapy.

Original languageEnglish (US)
Pages (from-to)15423-15428
Number of pages6
JournalBiochemistry
Volume41
Issue number51
DOIs
StatePublished - Dec 24 2002

ASJC Scopus subject areas

  • Biochemistry

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    Paoletti, A. C., Shubsda, M. F., Hudson, B. S., & Borer, P. N. (2002). Affinities of the nucleocapsid protein for variants of SL3 RNA in HIV-1. Biochemistry, 41(51), 15423-15428. https://doi.org/10.1021/bi026307n