Adenovirus knob trimers as tailorable scaffolds for nanoscale assembly

Mathew M Maye, Paul Freimuth, Oleg Gang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The use of proteins derived from Adenovirus, whose knob fragments trimeric structure and relative small size allow its use as the multidentate assembly agent with gold nanoparticles, was described. The mutant proteins were expressed in E. Coli and shown by gel electrolysis to assemble into SDS-resistance trimeric quaternary conformations. The DLS results suggest a relative polydisperse aggregate population, which is also manifested in loss SP-band extinction, due to increased scattering components and screened Au dilution extinction. The unique phenomena of the knob-1 system revealed an isolated nanoparticle phase, which consist of only knob-1 encapsulated Au. The nonmonotonic dependence of knob-Au assembly on concentration ratios is determined by the competition between the knob's mono and trivalent binding, which depends on the number of available sites on the nanoparticles.

Original languageEnglish (US)
Pages (from-to)1941-1944
Number of pages4
JournalSmall
Volume4
Issue number11
DOIs
StatePublished - Nov 2008
Externally publishedYes

Fingerprint

Knobs
Scaffolds (biology)
Adenoviridae
Scaffolds
Nanoparticles
Electrolysis
Mutant Proteins
Gold
Proteins
Gels
Escherichia coli
Dilution
Conformations
Population
Scattering

Keywords

  • Biomimetics
  • Nanoparticles
  • Proteins
  • Scaffolds
  • Self-assembly

ASJC Scopus subject areas

  • Biotechnology
  • Engineering (miscellaneous)
  • Biomaterials
  • Medicine(all)

Cite this

Adenovirus knob trimers as tailorable scaffolds for nanoscale assembly. / Maye, Mathew M; Freimuth, Paul; Gang, Oleg.

In: Small, Vol. 4, No. 11, 11.2008, p. 1941-1944.

Research output: Contribution to journalArticle

Maye, Mathew M ; Freimuth, Paul ; Gang, Oleg. / Adenovirus knob trimers as tailorable scaffolds for nanoscale assembly. In: Small. 2008 ; Vol. 4, No. 11. pp. 1941-1944.
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