TY - JOUR
T1 - Adamantane-platinum conjugate hosted in β-cyclodextrin
T2 - Enhancing transport and cytotoxicity by noncovalent modification
AU - Prashar, Deepali
AU - Shi, Yi
AU - Bandyopadhyay, Debjyoti
AU - Dabrowiak, James C.
AU - Luk, Yan Yeung
N1 - Funding Information:
We thank NSF–CAREER (# 0845686), and Syracuse Center of Excellence for CARTI award supported by the U.S. Environmental Protection Agency (grants Nos.: X-83232501-0) for financial support.
PY - 2011/12/15
Y1 - 2011/12/15
N2 - This work reports the synthesis of a complex of a carboplatin analog having tethered adamantane that is encapsulated in the hydrophobic cavity of β-cyclodextrin (βCD) and its cytotoxic activity towards human neuroblastoma cells (SK-N-SH). We found that this inclusion complex of βCD adamantane carboplatin analog exhibited higher cytotoxicity towards SK-N-SH cells than carboplatin itself, and the inclusion complex exhibited a higher binding to plasmid pBR322 deoxyribonucleic acid (DNA) than carboplatin. Confocal fluorescence images of SK-N-SH cells treated with βCD having an attached fluorescein isothiocyanate (FITC)-tag exhibited fluorescence in the vicinity of the nuclei of the neuroblastoma cells. Direct measurements of the platinum content in SK-N-SH cells using inductively coupled plasma mass spectrometry (ICP-MS) indicated that the uptake rate of carboplatin was about 4 times higher than βCD adamantane carboplatin analog inclusion complex. When compared to carboplatin, we believe that the higher cytotoxicity of inclusion complex towards SK-N-SH cells is due to its higher DNA binding ability as compared to carboplatin, and more efficient delivery to the nucleus of the cell. This work suggests that the advantage of deliberate noncovalent modification with βCD through host-guest chemistry may also be broadly applicable to other anticancer agents as well.
AB - This work reports the synthesis of a complex of a carboplatin analog having tethered adamantane that is encapsulated in the hydrophobic cavity of β-cyclodextrin (βCD) and its cytotoxic activity towards human neuroblastoma cells (SK-N-SH). We found that this inclusion complex of βCD adamantane carboplatin analog exhibited higher cytotoxicity towards SK-N-SH cells than carboplatin itself, and the inclusion complex exhibited a higher binding to plasmid pBR322 deoxyribonucleic acid (DNA) than carboplatin. Confocal fluorescence images of SK-N-SH cells treated with βCD having an attached fluorescein isothiocyanate (FITC)-tag exhibited fluorescence in the vicinity of the nuclei of the neuroblastoma cells. Direct measurements of the platinum content in SK-N-SH cells using inductively coupled plasma mass spectrometry (ICP-MS) indicated that the uptake rate of carboplatin was about 4 times higher than βCD adamantane carboplatin analog inclusion complex. When compared to carboplatin, we believe that the higher cytotoxicity of inclusion complex towards SK-N-SH cells is due to its higher DNA binding ability as compared to carboplatin, and more efficient delivery to the nucleus of the cell. This work suggests that the advantage of deliberate noncovalent modification with βCD through host-guest chemistry may also be broadly applicable to other anticancer agents as well.
KW - Anticancer agents
KW - Cyclodextrins
KW - DNA
KW - Host-guest chemistry
KW - Noncovalent modification
KW - Solvation
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U2 - 10.1016/j.bmcl.2011.10.006
DO - 10.1016/j.bmcl.2011.10.006
M3 - Article
C2 - 22050886
AN - SCOPUS:81255154461
SN - 0960-894X
VL - 21
SP - 7421
EP - 7425
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 24
ER -