Activation of myosin V-based motility and F-actin-dependent network formation of endoplasmic reticulum during mitosis

Torsten Wöllert, Dieter G. Weiss, Hans Hermann Gerdes, Sergei A. Kuznetsov

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

It is widely believed that microtubule- and F-actin-based transport of cytoplasmic organelles and membrane fusion is down-regulated during mitosis. Here we show that during the transition of Xenopus egg extracts from interphase to metaphase myosin V-driven movement of small globular vesicles along F-actin is strongly inhibited. In contrast, the movement of ER and ER network formation on F-actin is up-regulated in metaphase extracts. Our data demonstrate that myosin V-driven motility of distinct organelles is differently controlled during the cell cycle and suggest an active role of F-actin in partitioning, positioning, and membrane fusion of the ER during cell division.

Original languageEnglish (US)
Pages (from-to)571-577
Number of pages7
JournalJournal of Cell Biology
Volume159
Issue number4
DOIs
StatePublished - Nov 25 2002
Externally publishedYes

Keywords

  • Cell cycle
  • Membrane fusion
  • Oocyte
  • Organelle movement
  • Xenopus

ASJC Scopus subject areas

  • Cell Biology

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