Abstract
The unicellular eukaryote Chlamydomonas reinhardtii is a phototactic alga that swims toward or away from light, using rhodopsin as the photopigment. The activity of retinal analogues was tested in the mutant FN68, which has high phototactic sensitivity only after incubation with retinal or analogues of retinal. Analogues prevented from isomerizing about the 7-ene, 9-ene, 11-ene, 13-ene, or 15-ene (C=N+H) bonds retained full activity. Also, bleaching, protonation of the N, and a stable geometrically altered chromophore are not required for full activity. An attractive hypothesis is that charge redistribution in the excited state of retinal directly triggers the activity of rhodopsin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 819-824 |
| Number of pages | 6 |
| Journal | Biochemistry |
| Volume | 28 |
| Issue number | 2 |
| State | Published - 1989 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Activation of Chlamydomonas rhodopsin in vivo does not require isomerization of retinal. / Foster, Kenneth W.; Saranak, Jureepan; Derguini, Fadila; Zarrilli, Gerald R.; Johnson, Randy; Okabe, Masami; Nakanishi, Koji.
In: Biochemistry, Vol. 28, No. 2, 1989, p. 819-824.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Activation of Chlamydomonas rhodopsin in vivo does not require isomerization of retinal
AU - Foster, Kenneth W.
AU - Saranak, Jureepan
AU - Derguini, Fadila
AU - Zarrilli, Gerald R.
AU - Johnson, Randy
AU - Okabe, Masami
AU - Nakanishi, Koji
PY - 1989
Y1 - 1989
N2 - The unicellular eukaryote Chlamydomonas reinhardtii is a phototactic alga that swims toward or away from light, using rhodopsin as the photopigment. The activity of retinal analogues was tested in the mutant FN68, which has high phototactic sensitivity only after incubation with retinal or analogues of retinal. Analogues prevented from isomerizing about the 7-ene, 9-ene, 11-ene, 13-ene, or 15-ene (C=N+H) bonds retained full activity. Also, bleaching, protonation of the N, and a stable geometrically altered chromophore are not required for full activity. An attractive hypothesis is that charge redistribution in the excited state of retinal directly triggers the activity of rhodopsin.
AB - The unicellular eukaryote Chlamydomonas reinhardtii is a phototactic alga that swims toward or away from light, using rhodopsin as the photopigment. The activity of retinal analogues was tested in the mutant FN68, which has high phototactic sensitivity only after incubation with retinal or analogues of retinal. Analogues prevented from isomerizing about the 7-ene, 9-ene, 11-ene, 13-ene, or 15-ene (C=N+H) bonds retained full activity. Also, bleaching, protonation of the N, and a stable geometrically altered chromophore are not required for full activity. An attractive hypothesis is that charge redistribution in the excited state of retinal directly triggers the activity of rhodopsin.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024977345&partnerID=8YFLogxK
M3 - Article
C2 - 2713348
AN - SCOPUS:0024977345
VL - 28
SP - 819
EP - 824
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 2
ER -