ACTION SPECTRA FOR PHOTOTROPIC BALANCE IN Phycomyces blakesleeanus: DEPENDENCE ON REFERENCE WAVELENGTH and INTENSITY RANGE

Paul Galland, Edward D. Lipson

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Abstract— Action spectra for phototropic balance of Phycomyces blakesleeanus sporangiophores were measured for various reference wavelengths and intensity ranges. Balance action spectra were made at fluence rates of 10‐4 W m‐2 with reference wavelengths of 450 nm, 394 nm, 507 nm, and broadband blue light. For broad‐blue light and 450 nm light as references, typical flavin‐like action spectra were found with a ma jor peak at 455 nm, a secondary peak at 477 nm, and a minor peak at 383 nm; these peaks are wider for broad blue than for 450 nm light. With the 394 nm reference, there is a major peak at 455 nm, a secondary peak at 477 nm and a minor peak at 394 nm. An action spectrum with 507 nm reference has a major peak at 455 nm and a minor peak at 383 nm, but no peak at 477 nm. A balance action spectrum was made with 450 nm reference light near threshold intensity (2 times 10‐8 W m‐2); there, the 386 nm peak is greatly reduced, while the 455 nm peak is enhanced. The intensity dependence of the 386 nm peak was studied in detail for reference light of 450 nm. We found that the relative quantum efficiency of the 386 nm light increases with the logarithm of the 450 nm fluence rate; in the high intensity range (0.3 W m‐2) the relative quantum efficiency of the 386 nm light is 1.3 and approaches zero at 10‐9 W m‐2. These findings indicate that P. blakesleeanus phototropism is mediated by multiple interacting pigments or by a photochromic photoreceptor.

Original languageEnglish (US)
Pages (from-to)323-329
Number of pages7
JournalPhotochemistry and photobiology
Volume41
Issue number3
DOIs
StatePublished - Mar 1985

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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