Actinomycin D induced DNase I hypersensitivity and asymmetric structure transmission in a DNA hexadecamer

Karl D. Bishop, Philip N. Borer, Yao qi Huang, Michael J. Lane

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

DNase I cleavage rates and nmr chemical shifts are shown to change for DNA sequences distal to an intercalated actinomycin D molecule in a duplex hexadecamer upon drug binding. Both sets of observations suggest that the source of these changes is a DNA-mediated structural response. The nmr results imply the response is transmitted preferentially in a 5′-to-3′ direction from the drug binding site. An inequivalent response of the two strands to a ligandinduced conformational change immediately suggests a mechanism for distinguishing the sense and antisense strands of DNA.

Original languageEnglish (US)
Pages (from-to)871-875
Number of pages5
JournalNucleic acids research
Volume19
Issue number4
DOIs
StatePublished - Feb 25 1991

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Actinomycin D induced DNase I hypersensitivity and asymmetric structure transmission in a DNA hexadecamer'. Together they form a unique fingerprint.

  • Cite this