Actin-dependent organelle movement in squid axoplasm

Sergei A. Kuznetsov, George M. Langford, Dieter G. Weiss

Research output: Contribution to journalArticle

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Abstract

STUDIES of organelle movement in axoplasm extruded from the squid giant axon have led to the basic discoveries of microtubule-dependent organelle motility1-3 and the characterization of the microtubule-based motor proteins kinesin and cytoplasmic dynein4,5. Rapid organelle movement in higher animal cells, especially in ,neurons, is considered to be microtubule-based. The role of actin filaments, which are also abundant in axonal cytoplasm6,7, has remained unclear. The inhibition of organelle movement in axoplasm by actin-binding proteins8-11 such as DNase I, gelsolin and synapsin I has been attributed to their ability to disorganize the microtubule domains where most of the actin-fi laments are located7. Here we provide evidence of a new type of organelle movement in squid axoplasm which is independent of both microtubules and microtubule-based motors. This movement is ATP-dependent, unidirectional, actin-dependent, and probably generated by a myosin-like motor. These results demonstrate that an actomyosin-like mechanism can be directly involved in the generation of rapid organelle transport in nerve cells.

Original languageEnglish (US)
Pages (from-to)722-725
Number of pages4
JournalNature
Volume356
Issue number6371
DOIs
StatePublished - Jan 1 1992

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