TY - JOUR
T1 - Actin-dependent organelle movement in squid axoplasm
AU - Kuznetsov, Sergei A.
AU - Langford, George M.
AU - Weiss, Dieter G.
PY - 1992
Y1 - 1992
N2 - STUDIES of organelle movement in axoplasm extruded from the squid giant axon have led to the basic discoveries of microtubule-dependent organelle motility1-3 and the characterization of the microtubule-based motor proteins kinesin and cytoplasmic dynein4,5. Rapid organelle movement in higher animal cells, especially in ,neurons, is considered to be microtubule-based. The role of actin filaments, which are also abundant in axonal cytoplasm6,7, has remained unclear. The inhibition of organelle movement in axoplasm by actin-binding proteins8-11 such as DNase I, gelsolin and synapsin I has been attributed to their ability to disorganize the microtubule domains where most of the actin-fi laments are located7. Here we provide evidence of a new type of organelle movement in squid axoplasm which is independent of both microtubules and microtubule-based motors. This movement is ATP-dependent, unidirectional, actin-dependent, and probably generated by a myosin-like motor. These results demonstrate that an actomyosin-like mechanism can be directly involved in the generation of rapid organelle transport in nerve cells.
AB - STUDIES of organelle movement in axoplasm extruded from the squid giant axon have led to the basic discoveries of microtubule-dependent organelle motility1-3 and the characterization of the microtubule-based motor proteins kinesin and cytoplasmic dynein4,5. Rapid organelle movement in higher animal cells, especially in ,neurons, is considered to be microtubule-based. The role of actin filaments, which are also abundant in axonal cytoplasm6,7, has remained unclear. The inhibition of organelle movement in axoplasm by actin-binding proteins8-11 such as DNase I, gelsolin and synapsin I has been attributed to their ability to disorganize the microtubule domains where most of the actin-fi laments are located7. Here we provide evidence of a new type of organelle movement in squid axoplasm which is independent of both microtubules and microtubule-based motors. This movement is ATP-dependent, unidirectional, actin-dependent, and probably generated by a myosin-like motor. These results demonstrate that an actomyosin-like mechanism can be directly involved in the generation of rapid organelle transport in nerve cells.
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U2 - 10.1038/356722a0
DO - 10.1038/356722a0
M3 - Article
C2 - 1570018
AN - SCOPUS:0026534466
SN - 0028-0836
VL - 356
SP - 722
EP - 725
JO - Nature
JF - Nature
IS - 6371
ER -